Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1994-04-28
1995-12-26
Ceperley, Mary E.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 75, 435975, 436531, 436815, 436825, G01N 33546, G01N 33577
Patent
active
054787297
ABSTRACT:
This invention pertains to methods to detect a compound in the presence of a homolog that is immunologically related to the analyte. The invention is particularly suited for the detection of homocysteine in the presence of cysteine. The methods of this invention involve chemically modifying both the analyte and the homolog to increase their immunogenicity and facilitate antibody recognition. More importantly, this modification is done to make these compounds immunologically distinct. Antibodies to the immunologically distinct compounds are then prepared. An assay protocol comprises chemically modifying the analyte and homolog and then immunochemically detecting the modified analyte by means of the aforementioned antibodies.
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Ueland, et al., Clinical Chemistry, vol. 39(9): 1764-1779 (1993), "Total Homocysteine in Plasma or Serum: Methods and Clinical Applications".
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Fiskerstrand, et al., Clinical Chemistry, vol. 39(2): 263-271 (1993), "Homocysteine and Other Thiols in Plasma and Urine: Automated Determination and Sample Stability".
Refsum, et al., Clinical Chemistry, vol. 31(4): 624-628 (1985), "Radioenzymic Determination of Homocysteine in Plasma and Urine".
Jue, et al., Analytical Biochemistry, vol. 210: 39-44 (1993), "Identification of Cysteine Residues Alkylated with 3-Bromopropylamine by Protein Sequence Analysis".
Goodman Thomas C.
Ullman Edwin F.
Van Atta Reuel B.
Ceperley Mary E.
Precivale Shelley G.
Syntex (U.S.A.) Inc.
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