Immunoassay for homocysteine

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving hydrolase

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

435 4, 435 15, 435 21, 435 71, 435 16, 435 23, 435 28, 435 24, C12Q 134, C12Q 100, C12Q 148, C12Q 142

Patent

active

059587172

ABSTRACT:
The invention relates to a method for assaying homocysteine in a sample such as blood, plasma or urine, which comprises the steps of contacting the sample with a homocystene conveying enzyme and at least one substrate for the enzyme other than homocysteine, and without chromatographic separation, assessing a non-labelled analyte selected from a homotysteine co-substrate and the homocysteine conversion products of the enzymic conversion of homocysteine by said enzyme.

REFERENCES:
patent: 4281061 (1981-07-01), Zuk et al.
patent: 4478934 (1984-10-01), Sato et al.
patent: 5124351 (1992-06-01), Rabinovitz et al.
patent: 5385933 (1995-01-01), Rabinovitz et al.
patent: 5478729 (1995-12-01), Van Atta et al.
patent: 5631127 (1997-05-01), Sundrehagen
patent: 5827645 (1998-10-01), Sundrehagen
Bergmeyer, Methods of Enzymatic Analysis, 1985, pp. 110-117 and 357-364 and 403-409.
Refsum et al., Clinical Chemistry, vol. 31, No. 4, Apr. 1985, pp. 624-628.
Refsum et al., "Radioenzymic Determination of Homocysteine in Plasma and Urine", Clin. Chem. 31(4), pp. 625-628 (1985).
Hemmila, "Fluoroimmunoassays and Immunofluorometric Assays", Clin. Chem. 31/3, pp. 359-370 (1985).
Harlow et al., "Antibodies a Laboratory Manual", Chapter 14, Cold Spring Harbor Laboratory, 1988.
Ueland et al., "Total Homocysteine in Plasma or Serum: Methods and Clinical Applications", Clin. Chem. 39/9, pp. 1764-1779 (1993).
Andersson et al., "Homocysteine and other Thiols determined in Plasma by HPLC and Thiol-Specific Postcolumn Derivatization", Clin. Chem. 39/8, pp. 1590-1597 (1993).

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Immunoassay for homocysteine does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Immunoassay for homocysteine, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Immunoassay for homocysteine will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-702154

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.