Immunoassay for H. pylori in fecal specimens

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

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4352521, G01N 33554, G01N 33569

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active

058719423

ABSTRACT:
A process for the determination of H. pylori in a fecal specimen which comprises; (a) collecting a smear of a fecal specimen on a substrate; (b) immersing at least the portion of the substrate carrying the smear in a sample diluent so as to disperse the fecal specimen in the diluent; (c) contacting the fecal specimen in the diluent with a first polyclonal antibody for H. pylori antigen to form a complex of the antibody and the antigen; (d) separating the specimen and the complex; (e) exposing the complex to a second polyclonal antibody for the antigen and a portion of the antibody reacting with the complex, one of said first and second antibody being bound to a solid carrier and the other being labelled with a detection agent; and (f) determining the amount of the labelled antibody and in turn determining the presence of H. pylori antigen in the fecal specimen.

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