Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1982-03-31
1984-05-22
Marantz, Sidney
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435293, 436506, 436507, 436529, 436531, 436538, 436539, 436809, G01N 3354
Patent
active
044502311
ABSTRACT:
An immunoassay of a specimen of a serum or the like to determine immune complexes, includes producing on a plastic base a layer of a non-proteinaceous, non-ionic polymer which will adhere to the plastic base and has the capability of absorbing immune complexes of the specimen, placing a specimen on the layer and treating the layer to produce an indication of the amount of immune complexes. The polymer may be polyethylene glycol, dextran, polyvinyl chloride, a polymeric polyol or an adduct of polyethylene glycol. Washing with conventional solutions, addition of an antihuman IgG coupled with an enzyme and addition of a substrate reactive therewith, are similar to the ELISA test, with color measurement as by spectrophotometer. Or, the addition of anti IgG-I.sup.125 and measurement by a scintillation counter may be used. The ethylene glycol may range in molecular weight from 2,000 to 20,000, although 6,000 to 8,000 is preferred. A product for use in such an assay is a plate having wells or a test tube formed of plastic, polystyrene and polyvinyl chloride being preferred, with a layer of such non-proteinaceous, non-ionic layer on the plate wells or the cavity of the test tube. This product, if it is not to be used immediately, should be provided with a layer or film protecting the polymer layer from the air.
REFERENCES:
patent: 4143124 (1979-03-01), Masson
patent: 4146640 (1979-03-01), Lenhard
patent: 4153417 (1979-05-01), Hallgren
patent: 4162895 (1979-07-01), Cambiaso
patent: 4233286 (1980-11-01), Soothill
"Manual of Clinical Immunology", N. R. Rose et al., eds., Chapt. 90, pp. 669-675, American Society for Microbiology, Washington, D.C., 1976.
Chemical Abstracts, 96: 179327u (1982).
Chemical Abstracts, 96: 120507z to 120514z (1982).
Berkstresser Jerry W.
Biostar Medical Products, Inc.
Marantz Sidney
Van Valkenburgh Horace B.
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