Immunoassay for detecting immunoglobulins and test kit

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

435 34, 435810, 436513, 436518, 436519, 436534, 436805, 436808, 436811, G01N 3353, G01N 33569

Patent

active

046122818

ABSTRACT:
An immunoassay for detecting disease associated Ig, such as IgM associated with acute forms of Toxoplasma gondii infection. Wells of microtiter plates are first coated with an antibody against the Ig to be detected and the test sample, e.g. serum, is then added and incubated. After removal of non-adsorbed materials from the wells, an insoluble visible form of the cognate antigen to the Ig, such as antigen fixed to latex particles, is added. If no such Ig is present in the sample, the antigen will appear as a smooth button on the bottom of the well; whereas if such Ig is present in significant quantity, the antigen will be captured by adsorbed antiIg-Ig complexes and distributed on the wall of the well according to the distribution of the antiIg-Ig complex thereon.

REFERENCES:
patent: 3415361 (1968-12-01), Chambliss
patent: 3562384 (1971-02-01), Arquilla
patent: 3853467 (1974-11-01), Giaever
patent: 3904367 (1975-09-01), Galikersuch
patent: 3979184 (1976-09-01), Giaever
patent: 3979509 (1976-09-01), Giaever
patent: 4041146 (1977-08-01), Giaever
patent: 4092116 (1978-05-01), Giaever
patent: 4115543 (1978-09-01), Wallace
patent: 4169138 (1979-09-01), Jonsson
patent: 4189466 (1980-02-01), Ainis
patent: 4292403 (1981-09-01), Duermeyer
patent: 4297004 (1981-10-01), Claude
patent: 4407943 (1983-10-01), Cole et al.
A. F. Bradburne et al., Journal of General Virology, 44, part 3, 615-623 (1979).
Palmer et al., Serodiagnosis of Toxoplasmosis, Rubella, Cytomegalic Inclusion Disease, Herpes Simplex, U.S. Dept. HEW PITS Center for Dis. Control, Atlanta, Ga., 1/74, pp. 24-37.
Desmont, "Direct Agglutination Test", J. Clin. Micro., 6/80, vol. 9, No. 6, pp. 562-568.
Desmont et al., "Immunoglobulin M-Immunosorbent Assay", J. Clin. Micro., 81, pp. 486-491, vol. 14, #5.

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Immunoassay for detecting immunoglobulins and test kit does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Immunoassay for detecting immunoglobulins and test kit, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Immunoassay for detecting immunoglobulins and test kit will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-1995531

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.