Immunoassay for complement fragments

Details Immunoassay for complement fragments Immunoassay for complement fragments

1986-11-03

1988-03-15

Moskowitz, Margaret

Chemistry: analytical and immunological testing

For preexisting immune complex or auto-immune disease

436536, 436538, 436539, 436540, 436542, 436809, 436821, 436825, G01N 33564, G01N 33536

Patent

active

047313366

ABSTRACT:
A method for assaying complement fragment C.sub.3 a, C.sub.4 a or C.sub.5 a or the des-Arg derivative thereof in a biological sample which comprises combining equal volumes of the biological sample and a solution of 0.8 to 1.6% of an acridine derivative selected from the group consisting of acrinol, acriflavine, acriflavine hydrochloride, and aminacrine, incubating the mixture for about one minute to 2 hour at about 25.degree. C., recovering the supernatant from the resultant precipitate, incubating the supernatant with a known amount of a labeled complement fragment selected from C.sub.3 a, C.sub.4 a or C.sub.5 a or the des-Arg derivative thereof and a known amount of antibody which recognizes said complement fragment or des-Arg derivative thereof, separating the free labeled complement fragment from the bound labeled complement fragment, measuring the amount of labeled complement fragment in either the free or antibody bound complement component, and determining the concentration of complement fragment or the des-Arg derivative thereof in the biological sample by comparison to a standard curve and a mercantil kit useful in performing said method.

REFERENCES:
patent: 3607857 (1971-09-01), Nelson
patent: 3745155 (1973-07-01), Dahlgren
patent: 3903254 (1975-09-01), Dahlgren
T. E. Hugli and D. E. Chenoweth, "Biologically Active Peptides of Complement: Techniques & Significance of C3a and C5a Measurements", Immunoassays: Clinical Lab. Techniques for the 1980's , A. R. Liss, Inc., New York, 1980, pp. 443-460.
M. Stastny and J. Horeisi, "The Interaction of Acridine Dyes with Blood Plasma Proteins", Clin. Chim. Acta, 6, 1961, pp. 782-793.
Hood, L. E., et al, Immunology, p. 49.
Benjamin Cummings Co., Menlo Park, Calif. (1978).
Baumgarten, A., Immunology, CRC Handbook Series Section F, pp. 89-116, CRC Press (1979).
Satoh, Paul S., Biotechnique, vol. 1(2), pp. 90-95 (1983).

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