Immunoassay for AH receptor transformed by dioxin-like compounds

Details Immunoassay for AH receptor transformed by dioxin-like compounds Immunoassay for AH receptor transformed by dioxin-like compounds

1993-07-27

1996-06-25

Parr, Margaret

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 721, 435 792, 435 793, 435 794, 435970, 435975, 436501, 436503, 436807, 436809, 422 681, 422 69, G01N 3353

Patent

active

055298990

ABSTRACT:
The present invention is directed to a method of detecting dioxin-like compounds in a test sample. The test sample is contacted with a heteromer formed from a plurality of proteins, one of which is an inactive Ah receptor. If dioxin-like compounds are present in the test sample, they will bind to the Ah receptor causing it to dissociate from the heteromer as a complex containing active Ah receptor bound to a dioxin-like compound ligand. The presence of the complex is then detected. The process of the present invention can be practiced utilizing solid phase capture, competitive, and sandwich immunoassay test kit formats.

REFERENCES:
patent: 5262297 (1993-11-01), Sutton
patent: 5270164 (1993-12-01), Anderson et al.
Harper et al Molecular Pharmacology 42 pp. 603-612 (1992) "In Vitro Transformation of the Human Ah Receptor and Its Binding to a Dioxin Response Element".
Gasiewicz et al Molecular Pharmacology 40 pp. 607-612 (1991) ".alpha.-Napthoflavone Acts as an Antagonist of 2,3,7,8-Tetrachloro-dibenzo-p-dioxin by Forming an Inactive Complex with the Ah Receptor".
Reyes et al Science 256 pp. 1193-1195, (22 May 1992) "Identification of the Ah Receptor Nuclear Translator Protein (Arnt) as a component of the DNA Binding Form of the Ah Receptor".
Poland et al Molecular Pharmacology 39 pp. 20-26 (1991) "characterization of Polyclonal Antibodies to the Ah Recepor Prepared by Immunization with a Synthetic Peptide Hapten".

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