Immunoassay employing optical pulse particle size analysis

Details Immunoassay employing optical pulse particle size analysis Immunoassay employing optical pulse particle size analysis

1986-06-06

1989-07-25

Warden, Robert J.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving virus or bacteriophage

435 7, 436517, 436518, 436520, 436523, 436529, 436531, 436533, 436805, 436815, 436816, 436817, 436823, 356336, 356338, C12Q 170, G01N 33543, G01N 33549, G01N 1502

Patent

active

048513296

ABSTRACT:
This invention provides a means for determining the concentration of any of a wide range of antibody or antigen molecules with a high degree of specificity, accuracy and sensitivity. Antigen or antibody concentration is determined by effecting an agglutination reaction in a liquid medium and determining the cluster size distribution of agglutinated particles by optical pulse particle size analysis. The measured cluster size distribution then is compared with a standard quantitative relationship between the cluster size distribution and concentration of the antigen or antibody being tested. By this means one may specifically ascertain the absolute concentration of the antigen or antibody in question in the sample being analyzed. In addition to detecting antigen or antibody molecules, the process of this invention can be used to determine the concentration of any substance capable of specifically promoting or inhibiting an agglutination reaction such as viruses, white blood cells or the like.

REFERENCES:
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Cohen, R. J. and Benedek, G. B., Immunochemistry 12:349 (1975).
von Schulthess, G. K. et al., Immunochemistry 13:955 (1976).
von Schulthess, G. K. et al., Immunochemistry 13:963 (1976).
von Schulthess, G. K. et al., Molecular Immunology 17:81 (1980).
"Experimental Analysis of Diffusion Controlled Coagulation Using An Optical Pulse Particle Size Analyzer", Bowen, M. S. et al., Kinetics of Aggregation and Gelation, (1984), pp. 185-190.
Bowen, et al., J. of Colloid and Interface Science, vol. 105 No. 2, p. 605, Jun. 1985.
Bowen, et al., J. of Colloid and Interface Science, vol. 105, No. 2, pp. 617-627, Jun. 1985.

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