Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1978-03-16
1981-01-06
Fagelson, Anna P.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
23230B, 424 8, 424 12, 424 13, G01N 3100, G01N 3114, G01N 3316
Patent
active
042437490
ABSTRACT:
According to the present invention there is provided a sensitive quantitative assay for the determination of a hapten "X" which comprises: preparing a conjugate of the hapten X which conjugate is adapted to ellicit antibody formation, and injecting a mammal with said conjugate, resulting in a specific antibody against the said hapten, "anti-X"; preparing a conjugate of the hapten X and of another entity Y, which is either another hapten or a larger molecule, injecting a mammal with the entity Y if it is a larger molecule, or with a conjugate thereof if it is a small molecule which does not by itself ellicit antibody formation, so as to form anti-Y antibodies, preparing a conjugate of a suitable enzyme and the antibody against Y, i.e. an (enzyme)-(anti-Y)-conjugate; adsorbing the antibodies against X, i.e. anti-X on a solid support, contacting said solid support with a mixture containing the unknown quantity of hapten X and a known quantity of the X-Y conjugate; removing unreacted X and X-Y, leaving all anti-X sites occupied, adding enzyme-labelled anti-Y antibody; adding a suitable substrate resulting in a color reacting and measuring the intensity of color indicating the quantity of bound enzyme and deducing from calibration curves the quantity of the hapten X; and to a kit for carrying out such assay.
REFERENCES:
patent: 3998943 (1976-12-01), Ullman
patent: 4017597 (1977-04-01), Reynolds
Feldmann et al., (Ed.), Immunoenzymatic Tech., Proc. 1st Int. Sym. on Immunoenzym, Tech., North Holland/Amer. Elsevier Pub. Co. N.Y., 1976 pp. 125-133.
Clin. Chem., vol. 22, 1976 pp. 1243-1255.
Hexter Charles S.
Sadeh Dora
Fagelson Anna P.
Hypolab S.A.
Miles Yeda Ltd.
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