Immunoassay element and process and immunoassay

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

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435 791, 435 792, 435 14, 435 18, 435 22, 435 25, 435 28, 435966, 435969, 435970, 436518, 436528, 436529, 436530, 436531, 436537, 436807, 436810, 422 56, 422 57, G01N 3353

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055695892

ABSTRACT:
An immunoassay element for quantitatively analyzing a ligand by determining the change in enzymatic activity caused by a reaction between the ligand, a linked product of the ligand and a high molecular weight compound and an enzyme-labelled antibody. The immunoassay element comprises a substrate layer containing a non-diffusible substrate which forms a diffusible material in the presence of the enzyme, and a reagent layer containing a fragmenting enzyme for further fragmenting the non-difussible material. Also provided is a process for quantitatively analyzing a ligand contained in a sample by the use of the immunoassay element.

REFERENCES:
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patent: 4376197 (1983-03-01), Wallenfels
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patent: 4782018 (1988-11-01), Eikenberry et al.
patent: 4945043 (1990-07-01), Gerber
Kaufman et al, Clinical Chemistry, vol. 26, No. 7, Jun. 1980, pp. 846-853.
N. Tietz, (ed.) Textbook of Clinical Chemistry (W B. Saunders Company 1986) pp. 729-734.

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