Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Reexamination Certificate
2005-09-19
2010-10-26
Cheu, Jacob (Department: 1641)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
C435S007910, C435S007920, C435S014000, C435S018000, C435S022000, C435S025000, C435S028000, C435S966000, C435S969000, C435S970000, C436S518000, C436S528000, C436S531000, C436S537000, C436S807000, C436S810000, C422S051000, C422S051000
Reexamination Certificate
active
07820402
ABSTRACT:
The immunoassay element for quantitatively analyzing an antigen by determining the change in enzymatic activity of an enzyme-labelled antigen or antibody caused by an immunological reaction. The immunoassay element comprises a substrate layer containing a non-diffusible substrate which forms a diffusible material in the presence of the labelling enzyme, and a reagent layer containing a fragmenting enzyme for further fragmenting the diffusible material into a lower molecular weight product. As the non-diffusible substrate, a substrate capable of reacting solely with the labelling enzyme and incapable of reacting the fragmenting enzyme is utilized. When an endo-active glucosidase is used as the labelling enzyme, and an exo-active glucosidase is used the fragmenting enzyme in the reagent layer, the non-diffusible substrate of the substrate layer is preferred to be an endo type selectively reactive substrate, which means a substrate having a reactivity specific to endo-active glucosidase. Highly sensitive assay is realized with high accuracy and high reproducibility and good storage stability.
REFERENCES:
patent: 5569589 (1996-10-01), Hiraoka et al.
Amano Yoshikazu
Ito Hitomi
Ito Toshihisa
Mori Toshihiro
Sakaino Yoshiki
Buckley Maschoff & Talwalkar LLC
Cheu Jacob
Do Pensee T
Fuji Photo Film Co. , Ltd.
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