Immunoassay device enclosing matrixes of antibody spots for cell

Details Immunoassay device enclosing matrixes of antibody spots for cell Immunoassay device enclosing matrixes of antibody spots for cell

1987-03-13

1989-05-09

Marantz, Sidney

Chemical apparatus and process disinfecting, deodorizing, preser

Analyzer, structured indicator, or manipulative laboratory...

Calorimeter

436532, 436807, 436809, 435298, 422 58, G01N 33543, G01N 148, C12M 122

Patent

active

048290106

ABSTRACT:
An immunoassay device comprising a support which has a substantially planar surface 14 on which is located an array of small, closely-spaced, discrete, antibody coated areas (spots) 17. A cover 16 is spaced from the support surface 14 and is positioned over the array of antibody coated spots 17. The cover is attached to the support surface to provide an enclosed chamber 30. In the cover there is at least one aperture communicating with the interior of the chamber 30 for introducing a liquid sample into the chamber. In addition, there is at least one additional aperture 34 in the cover, which also communicates with the interior of the chamber to allow air to escape upon introduction of the sample into the chamber. When a cell sample is introduced into the chamber it immediately fills the chamber and cells will settle uniformly over the matrix. The antibody-coated spots function as tiny immunoadsorbents for cells bearing antigens recognized by the antibody. The device can be used to determine the proportion of cells in a sample cell population.

REFERENCES:
patent: 3165450 (1963-03-01), Scheidt
patent: 4055394 (1977-10-01), Friedman et al.
patent: 4154795 (1979-05-01), Thorne
patent: 4216245 (1980-08-01), Johnson
patent: 4228127 (1980-10-01), Acevedo et al.
patent: 4315907 (1979-10-01), Fridlender et al.
patent: 4317810 (1982-03-01), Halbert et al.
patent: 4357142 (1982-11-01), Shall, Jr. et al.
patent: 4468371 (1984-08-01), Chen et al.
patent: 4471056 (1984-09-01), Grumet et al.
patent: 4591570 (1983-02-01), Chang
patent: 4647430 (1987-03-01), Zweig
patent: 4708933 (1984-06-01), Huang et al.
patent: 4761381 (1988-08-01), Blatt et al.
Tse Wen Chang, "Binding of Cells to Matrixes of Distinct Antibodies Coated on Solid Surface", 1983, pp. 217-223.
Paul Herbrink, Frans J. Van Bussel & Sven O. Warnaar, "The Antigen Spot Test (AST): A Highly Sensitive Assay for the Detection of Antibodies", 1981, pp. 293-298.
Langone et al., EDSl, "Immunochemical Techniques", Part C., pp. 90-105, by Sedlacek, H. H., et al., 1981.
Eva Engvall & Peter Perlmann, "Enzyme-Linked Immunosorbent Assay, Elisa", 1971, pp. 129-135.
Richard Hawkes, Evelyn Niday, & Julian Gordon, "A Dot-Immunobinding Assay for Monoclonal & Other Antibodies", 1981, pp. 142-147.
Michaelsen et al., Chemical Abstracts: 97:37269Y (1982). Abstract of "Sheep, Rabbit and Chicken Antisera Against a Human V.sub.H Fragment: Reactivity with Immunoglobins and Lumphocytes".

Affiliated with

Also associated with

Rating

Immunoassay device enclosing matrixes of antibody spots for cell does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Immunoassay device enclosing matrixes of antibody spots for cell, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Immunoassay device enclosing matrixes of antibody spots for cell will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-89878