Chemistry: physical processes – Physical processes – Crystallization
Patent
1978-01-23
1979-11-20
Marcus, Michael S.
Chemistry: physical processes
Physical processes
Crystallization
23915, 356341, 422 73, 250574, 424 12, 435 7, G01N 2124, G01N 3316
Patent
active
041749529
ABSTRACT:
Method and apparatus for determining the concentration of any of a wide range of antigen or antibody molecules with a high degree of specificity, accuracy and sensitivity. Antigen or antibody concentration is determined by effecting an agglutination reaction between carrier particles in a liquid medium, exposing the liquid medium to a beam of light, and measuring the ratio of the intensities of light scattered at two different angles as a function of antigen or antibody concentration. The unknown concentration of antibody or antigen is determined by comparison with similar measurements of the anisotropy ratio using known concentrations of the molecule being tested for. The agglutination reaction is performed using carrier particles coated with an agglutinant. The carrier particles should be fairly uniform in size and their diameter can be less than or comparable to the wavelength of light. By this invention, one may specifically ascertain the absolute concentration of the antigen or antibody in question in the sample being analyzed. In addition to detecting antigen or antibody molecules, the process of this invention can be used to determine the concentration of any substance capable of specifically promoting or inhibiting an agglutination reaction.
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Tengerdy et al.; Reaction Kinetic Studies of the Antigen-Antibody Reactor, Nature, 5/14/66, pp. 208-210, vol. 210.
Benedek George B.
Cannell David S.
Cohen Richard J.
Giglio Marzio
von Schulthess Gustav K.
Cook Paul J.
Marcus Michael S.
Massachusetts Institute of Technology
Smith, Jr. Arthur A.
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