Immunoassay apparatus

Radiant energy – Luminophor irradiation – With ultraviolet source

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Details

2504581, 436172, G01J 500, G01N 3353, G01N 33533

Patent

active

050327305

ABSTRACT:
In an immunoassay apparatus, a washing liquid containing a substrate is applied to a labeled antigen or a labeled antibody. The fluorescent substance formed from the substrate is exposed to stimulating rays, which cause it to produce the fluorescene, and the amount of the fluorescence is measured, whereby the amount of a specific antigen or a specific antibody in a liquid sample carried on a support is determined. A stimulating ray irradiating device guides stimulating rays from a stimulating ray source to the support. A first photodetector monitors the amount of the stimulating rays, and a monitor optical device guides part of the stimulating rays thereto. A second photodetector detects the amount of fluorescence produced by the fluorescent substance, and a fluorescence receiving device guides the fluorescence from the support to the second photodetector. The amount of the fluorescence detected by the second photodetector is normalized with the amount of the stimulating rays detected by the first photodetector. The first and second photodetectors have approximately identical temperature-photodetection sensitivity characteristics.

REFERENCES:
patent: 4133873 (1979-01-01), Noller
patent: 4222743 (1980-09-01), Wang
patent: 4279506 (1981-07-01), Maines
patent: 4447546 (1984-05-01), Hirschfeld
patent: 4498782 (1985-02-01), Proctor et al.
patent: 4931384 (1990-06-01), Layton et al.
patent: 4980278 (1990-12-01), Yamada et al.
Flore, M. et al., "The Abbott IMx Automated Benchtop Immunochemistry Analyzer System", Clinical Chemistry, vol. 34, No. 9, (1988), pp. 1726-1732.

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