Immunoassay and reagent kit used therefor

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

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435 792, 435 793, 435 794, 435 795, 435 15, 435975, 436536, C12Q 100, C12Q 148, G01N 33536

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active

053668618

ABSTRACT:
An immunoassay method performed in solution wherein a protein having an average molecular weight of 16,000 to 50,000 and an isoelectric point of 1.0 to 5.0 or a mixture containing the same is permitted to exist as the antigen-antibody reaction controller in the immunoreaction solution, and the final concentration of the antigen-antibody controller in the immunoreaction solution is controlled to 0.02 to 0.9% by weight, and a reagent kit to be used for an immunoassay containing the above antigen-antibody reaction controller at a final concentration of 0.02 to 0.9% by weight in the immunoreaction solution, as a part of the constituents thereof.

REFERENCES:
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Kuroki et al. Ped. Res. 19 (1985) 1017-1020.
Baldo et al. J. Bioch. Bioph. Meth. 12 (1986) 271-279.
Hybridoma, vol. 9, No. 2, 1990, "Determination of Antigenic Epitopes Recognized by Four Monoclonal Antibodies to Glutathione S-Transferase".
Supplementary European Search Report.
Choi et al, "Isolation and Characterization of a 35000 Molecular Weight Subunit Fetal Cartilage Matrix Protein," The Journal of Biological Chemistry, vol. 258, No. 1, pp. 655-661, Jan. 1983.
Journal of Clinical Microbiology, vol. 25, No. 3 (Mar., 1987) pp. 509-515.

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