Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1983-05-18
1986-05-06
Warden, Robert J.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 23, 436510, 436537, 436814, 436817, G01N 3353, G01N 33542, C12Q 138
Patent
active
045872120
ABSTRACT:
There is described an enzyme immunoassay for measuring the concentration of an analyte in a sample wherein the substrate for the enzyme forms at least a part of the sample. In a particular embodiment the sample comprises or consists of a milk sample and the enzyme is an enzyme capable of clotting milk. An example given of such an enzyme is chymosin. The assay described may be used to measure the concentration of progestagens or oestrogens in milk using the techniques of heterogeneous or homogeneous enzyme immunoassay. The results of such an assay give an indication of the fertility of a milk producing domestic animal (e.g. a cow) and may be used to diagnose pregnancy of such an animal. Particular compounds for use in the assay are described, as is a kit of reagents for use in the assay.
REFERENCES:
patent: 3817837 (1974-06-01), Rubenstein et al.
patent: 3826616 (1974-07-01), Laing
patent: 4294922 (1981-10-01), Heap
patent: 4463090 (1984-07-01), Harris
Chemical Abstracts, vol. 95, 1981, Abstract No. 111258w, Sauer et al.
Chemical Abstracts, vol. 95, 1981, abstract No. 2949b, Arnstadt et al.
Sauer et al, "The Use of Enzyme Immunoassay for the Measurement of Hormones with Particular Reference to the Determination of Progesterone in Unextracted Whole Milk, " in: Wardley et al, The ELISA: Enzyme Linked Immunosorbent Assay in Veterinary Research and Diagnosis, (Boston, Martinus Nijhoff, 1982) pp. 271-301.
Boots-Celltech Diagnostics Limited
Deck Randall E.
Warden Robert J.
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