Immunoassay

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

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Details

435184, 435810, 436510, 436537, 436814, 436817, G01N 33542, C12N 999

Patent

active

047161094

ABSTRACT:
There is described an enzyme inhibitor labelled immunoassay for measuring the concentration of an analyte in a sample wherein the substrate for the enzyme forms at least a part of the sample. In a particular embodiment the sample comprises or consists of a milk sample and the inhibitor label is capable of inhibiting the activity of an enzyme capable of clotting milk. Examples are given of suitable inhibitors. The assay described may be used to measure the concentration of progestogens or oestrogens in milk using the techniques of heterogeneous or homogeneous enzyme inhibitor labelled immunoassay. The results of such an assay give an indication of the fertility of a milk producing domestic animal (e.g. a cow) and may be used to diagnose pregnancy of such an animal. Particular compounds for use in the assay are described, as is a kit of reagents for use in the assay.

REFERENCES:
patent: 3817837 (1974-06-01), Rubenstein et al.
patent: 3826616 (1974-07-01), Laing
patent: 4134792 (1979-01-01), Boguslaski et al.
patent: 4294922 (1981-10-01), Heap
patent: 4463090 (1984-07-01), Harris
patent: 4587212 (1986-05-01), Baker
Friedrich et al. (1975), Occurence of Alpha Toxins in Raw Milk, Chem. Abstrts., 83(1): 1979d.
Sauer et al. (1981), Direct Enzyme Immunoassay of Progesterone in Bovine Milk, Chem. Abstr., 95: 1112580u.

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