Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Enzymatic production of a protein or polypeptide
Patent
1986-12-19
1989-07-25
Weimar, Elizabeth C.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Enzymatic production of a protein or polypeptide
4351723, 43524027, 435320, 530387, 935 95, 935104, 935108, C12P 2100, C12N 1500, C12N 500, C07K 1300
Patent
active
048513415
ABSTRACT:
A process is disclosed for purifying a recombinant fusion protein having an N-terminal identification sequence comprising multiple anionic amino acid residues, comprising forming a complex of the protein with a divalent cation dependent monoclonal antibody specific for the sequence, isolating the complex, and dissociating antibody and protein by selectively depleting the concentration of divalent cations in contact with the complex. A particular calcium-dependent monoclonal antibody, 4E11, is useful in an embodiment of the process which employs the identification peptide DYKDDDDK.
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Hopp Thomas P.
Prickett Kathryn S.
Hallquist Scott G.
Immunex Corporation
Mays Thomas D.
Weimar Elizabeth C.
Wight Christopher L.
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