Drug – bio-affecting and body treating compositions – Designated organic active ingredient containing – Peptide containing doai
Patent
1982-12-14
1987-06-09
Wiseman, Thomas G.
Drug, bio-affecting and body treating compositions
Designated organic active ingredient containing
Peptide containing doai
530350, 530370, 4241951, A61K 3578, A61K 3700
Patent
active
046720537
ABSTRACT:
Antiviral proteins phytolaccin.sub.1 and phytolaccin.sub.2 were isolated from the higher plant Phytolaccin americana using the technique of immunoaffinity chromatography. Phytolaccin proteins were purified to apparent homogeneity in a rapid and efficient chromatographic procedure utilizing immobilized monospecific anti-phytolaccin antibodies. Immunoaffinity-purified phytolaccin.sub.1 and phytolaccin.sub.2 were determined by denaturing gel electrophoresis to be of approximately 25,600 and 28,400 daltons, respectively. The two immunoaffinity-purified proteins were equally potent inhibitors of eukaryotic cell-free protein biosynthesis and exhibited mostly similar high-order UV derivative spectra. Antibodies against phytolaccin.sub.1 and phytolaccin.sub.2 did not cross-react with the heterologous antigen, indicating a structural dissimilarity between the two phytolaccin proteins.
Phytolaccin.sub.1 protein, purified according to the process of the invention, has been found to be an effective antiviral agent for the treatment of Herpes Simplex type II infections.
REFERENCES:
patent: 4195074 (1980-03-01), Safford, Jr.
Livingston, David from Meth. Enzymol. vol. XXXIV Affinity Techniques 1974 Edited by Jukoby et al. Academic Press NY pp. 723-731.
Irvin Archives of Biochem and Biophys V164 pp. 522-528 1972.
Grasso et al. Phytopathology V.68 (2) pp. 199-205 "Isolation and Partial Characterization of Virus Inhibitors from Plant Species Taxonomically Related to Phytolacca".
Aron et al. Antimicrob Agents and Chemotherapy Jun. 1980 pp. 1032-1033 V.17 (6) "Inhibition of Herpes Simplex Virus Multiplication by the Pokeweed Antiviral Protein".
Sutherland Science V. 132 Oct. 28, 1960 pp. 1252-1253 "Immunological Technique for Protein Isolation".
Irvin et al. Chem. Abst. V.93 1980 No. 2370g "Purification and Properties of a Second Antiviral Protein from Phytolacca americana which Inhibits Eukaryotic Ribosomer".
Chemical Abstracts vol. 88 101733p 1978 Salvatore Grasso.
Chemical Abstracts vol. 89 204192m 1979 Van den Berghe et al.
Chemical Abstracts vol. 86 183054p 1977 Michael Ussery.
Chemical Abstracts vol. 96 210521d 1981 Masuho et al.
Teskin Robin Lyn
Wiseman Thomas G.
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