Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
2007-04-03
2007-04-03
Le, Long V. (Department: 1641)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C435S004000, C435S069100, C435S024000, C435S174000, C436S518000, C436S089000, C436S501000, C216S036000, C216S036000, C216S036000
Reexamination Certificate
active
10175486
ABSTRACT:
The invention provides methods for isolating a modified peptide from a complex mixture of peptides, the method comprising the steps of: (a) obtaining a proteinaceous preparation from an organism, wherein the preparation comprises modified peptides from two or more different proteins; (b) contacting the preparation with at least one immobilized modification-specific antibody; and (c) isolating at least one modified peptide specifically bound by the immobilized modification-specific antibody in step (b). The method may further comprise the step of (d) characterizing the modified peptide isolated in step (c) by mass spectrometry (MS), tandem mass spectrometry (MS—MS), and/or MS3analysis, or the step of (e) utilizing a search program to substantially match the spectra obtained for the modified peptide during the characterization of step (d) with the spectra for a known peptide sequence, thereby identifying the parent protein(s) of the modified peptide. Also provided are an immunoaffinity isolation device comprising a modification-specific antibody, and antibodies against novel UFD1 and PTN6 phosphorylation sites.
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Graves, et al.,Pharmacol. Ther. 82: 111-21 (1999).
Wettenhall, et al.,Methods Enzymol. 201 186-199 (1991).
DeCorte, et al., “Identification of Tyr438 as the Major in citro c-src Phoosphorylation Site in Human Gesolin: a Mass Spectrometric Approach,” Prot. Sci. 8:234-241 (1999).
Kalo, et al., “Multiple In Vivo Tyrosine Phosphorylation Sites in EphB Receptors,”Biochem. 38: 14396-408 (1999).
Marcus, et al.,Electrophoresis21: 2622-2635 (2000).
Mann, et al..,Trends in Biotech. 20: 261-268 (2000).
Yu, et al., Epitope Mapping of Monoclonal Antibodies by Mass Spectrometry: Identification of Protein Antigens in Complex Biological Systems,J.A.m. Soc. Mass. Spec. 9: 208-215 (1998).
Mann, et al., “Analysis of Proteins and Proteomes by Mass Spectrometry,”Ann. Rev. Biochem. 70: 437-73 (2001).
Posewitz, et al.,Anal. Chem. 15: 2883-2892 (1999).
Mann, et al., “Quantitaative Proteomics,”Nat. Biotech. 17: 954-55 (1999).
Pandey, et al., “Identification of a Novel Immunoreceptor Tyrosine-Based Activation Motif-Containing Molecule, STAM2, by Mass Spectrometry and Its Involvement in Growth Factor and Cytokine Receptor Signaliing Pathways,”J. Biol. Chem. 49: 38633-639 (2000).
Steen, et al., “Detection of Tyrosine Phosphorylated Peptides by Precursor Ion Scanning Quadropole TOF Mass Spectrometry in Positive Ion Mode,”Anal. Chem. 73: 1440-448 (2001).
Haley, et al., AACR Meeting Poster Presentation: “Probing EGFr Signaling in HN5 Squamous Carcinoma Using the Quinazoline EGFr Inhibitor OSI-774 and Coupled Affinity Chromatography and Mass Spectrometry,” (May 2001) (www.aacr.org).
Gaitlin, et al., “Protein Identification at the Low Femtomole Level from Silver-Stained Gels Using a New Fritless Electrospray Interface for Liquid Chromotography Microspray and Nanospray Mass Spectrometry,”Anal. Biochem. 263: 93-101 (1998).
Erdument-Bromage, et al., “Examination of Micro-tip Reversed-Phase Liquid Chromatographic Extraction of Peptide Pools for Mass Spectrometric Analysis,”J. Chromatogr. A826:167-181 (1998).
Chirica, et al.,Anal. Chem. 72: 3605-3610 (2000).
Brumell, et al.,J. Bio. Chem. 272 875-882 (1997).
Blaukat, et al.,J. Biol. Chem. 276: 40431-40 (2001).
Prat, et al.,Naurology53: 2087-2092 (1999).
Tomaino & Rush, et al., “Phosphopeptide Detection by a Data-dependent, Neutral-loss Driven MS3 Scan Using Ion rap Mass Spectrometry”, Abstract ThOE 3:00, 50thASMS Conference on Mass Spectrometry and Allied Topics (Jun. 2002).
Raska, et al., “Direct MALDI-MS/MS of Peptides Bound to Affinity Media”, Abstract WPA 034, 50thASMS Conference on Mass Spectrometry and Allied Topics (Jun. 2002).
Gielbert, et al., “On-Line Immunoaffinity Extraction Liquid Chromatography Mass Spectrometry with Monolithic Supports”, Abstract MPA: 029, 50thASMS Conference on Mass Spectrometry and Allied Topics (Jun. 2002).
Novagen Technical Bulletin, “pET System Man
Comb Michael J.
Rush John
Tan Yi
Zha Xiangming
Zhang Hui
Cell Signaling Technology, Inc.
Cullem, Esq. James G.
Do Pensee T.
Le Long V.
Warner, Esq. Andrew J.
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