Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Reexamination Certificate
2007-07-10
2007-07-10
Le, Long V. (Department: 1641)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
C435S069100, C435S069700, C436S544000, C436S056000, C530S300000, C530S327000, C530S328000, C530S402000, C536S023400
Reexamination Certificate
active
11148074
ABSTRACT:
Disclosed is a method of isolating, purifying, or concentrating a target compound. The method includes the steps of onjugating the target compound to an epitope for a polyol-responsive monoclonal antibody (PR-mAb) to yield a conjugate; and then contacting the conjugate to an immunoaffinity matrix comprising a PR-mAb specifically reactive with the PR-mAb epitope. Preferred epitopes for use in the method include amino acids of from 4 to about 30 amino acids, wherein the amino acid sequence comprises the sub-sequence D-X-S-R, (where X is any natural or unnatural amino acid), such as TKDPSRVG and TQDPSRVG. Additional epitopes for use in the method include SLAELLNGLGGS and PTSPSYSPTSPSYS. The method enables the rapid isolation of desired target compounds under gentle purification conditions.
REFERENCES:
Corden et al. A unique structure at the carboxyl terminus of the largest subunit of eukaryotic RNA polymerase II. PNAS 1985, vol. 82, pp. 7934-79-38.
Hisatake et al. Conserved structural motifs between Xenopus and human TFIIB. Nucleic Acids Research. 1991, vol. 19, p. 6639.
Ing et al. Members of the steroid hormone receptor superfamily interact with TFIIB (S300-II). Journal of Biological Chemistry 1992, vol. 267, No. 25, pp. 17617-17623.
L.C. Anthony, R.R. Burgess, Conformational flexibility in sigma70 region 2 during transcription initiation,J. Biol. Chem.277 (2002) 46433-46441.
Anthony, L.C. Foley, K. Thompson, N. and Burgess R. R. Expression, purification of, and monoclonal antibodies to the sigma factors from E. coli.Methods Enzymol., 370 (2003) 181-192.
T. M. Arthur, R. R. Burgess, Localization of a sigma 70 binding site on the terminus of the /Escherichia coli/ RNA polymerase b' subunit,J. Biol. Chem.273 (1998) 31381-31387.
Bergendahl, V., Thompson, N.E., Foley, K., Olson, B., and Burgess, R.R. A cross-reactive polyol-responsive monoclonal antibody useful for isolation of core RNA polymerase from many bacterial species.Protein Expression Purification, 31(2003) 155-160.
R. R. Burgess, J. J. Jendrisak, A procedure for the rapid, large-scale purification of /Escherichia coli/ DNA-dependent RNA polymerase involving Polymin P precipitation and DNA-cellulose chromatography,BIochemistry21 (1975) 4634-4638.
R. R. Burgess, T. M. Arthur, B. C. Pietz, Mapping protein-protein interaction domains using ordered fragment ladder far-western analysis of hexahistidine-tagged fusion proteins,Methods Enzymol.328 (2000) 141-157.
R. R. Burgess, N.E. Thompson, Advances in gentle immunoaffinity chromatography,Curr Opin. Biotechnol.13 (2002) 304-308.
M. Chalfie, Y. Tu, G. Euskirchen, W. W. Ward, D. C. Prasher, Green fluorescent protein as a marker for gene expression,Science263 (1994) 802-805.
P. Cramer, D. A. Bushnell, J. Fu, A. L. Gnatt, B. Maier-Davis, N. E. THompson, R. R. Burgess, A. M. Edwards, P. R. David, R. D. Kornberg, Architecture of RNA polymerase II and implications for the transcription mechanism,Science288 (2000) 640-649.
Duellman, s. j., thompson, n. e. and Burgess, R. R. An epitope tag derived from human transcription factor IIB (TFIIB) that reacts with a polyol-responsive monoclonal antibody.Prot. Express Purific. 35 (2004) 147-155.
A.M. Edwards, S.A. Darst, W.J. Feaver, N.E. Thompson, R.R. Burgess, R.D. Kornberg, Purification and lipid-layer crystallization of yeast RNA polymerase II,Proc Natl Acad Sci USA87 (1990) 2122-2126.
Harlow E. and Lane D.: Antibodies: A Laboratory Manual. New York: Cold Spring Harbor Press, 1988.
Hill C. L., Bartholomew R., Beidler D., and David G.S., “Switch” immunoaffinity chromatography with monoclonal antibodies,Biotechniques, 1 (1983) 14-17.
J. W. Jarvik, C.A. Telmer, Epitope tagging,Ann Rev Genet32 (1998) 601-618.
J. J. Jendrisak, R. R. Burgess, A new method for the large-scale purification of wheat germ RNA polymerase II,Biochemistry14 (1975) 4639-4645.
Y. Jiang, S.J. Zhang, S.M. Wu, M.Y. Lee, Immunoaffinity purification of DNA polymerase delta,Arch Biochem Biophys320 (1995) 297-304.
Largaespada D.A., Jackson M. W., Thompson N. E., Kaehler D. A., Byrd L.G., and Mushinski J.F., The ABL-MYC retrovirus generates antigen-specific plasmacytomas by in vitro infection of activated B-lymphocytes from spleen and other murine lymphoid organs.J Immunol Methods, 197 (1996) 85-95.
N.A. Lynch, H. Jiang, D.T. Gibson, Rapid purification of the oxygenase component of toluene di-oxygenase from a polyol-responsive monoclonal antibody,Appl Environ Microbiol62 (1996) 2133-37.
P.L. Nagy, J. Griesenbeck, R.D. Kornberg, M.L. Cleary, A trithorax-group complex purfied from /Saccharomyces cerevisiae/ is required for methylation of histone H3,Proc Natl Acad Sci USA99 (2002) 90-94.
L. Rao, D. P. Jones, L. H. Nguyen, S. A. McMahan, R. R. Burgess, Epitope mapping using histidine-tagged protein fragments: application to /Escherichia coli/RNA polymerase sigma 70,Anal. Biochem.241 (1996) 173-179.
Rhemrev-Boom M.M., Yates M, Rudolph M., and Raedts M., Immunoaffinity chromatography: a versatile tool for fast and selective purification, concentration, isolation and analysis,J Pharm&Biomed Anal, 24 (2001) 825-833.
A.B. Sparks, N.B. Adey, S. Cwirla, B.K. Kay, Screening phage-displayed random peptide libraries, in B.K. Kay, J. Winter, and J. McCafferty (Eds.) Phage Display of Peptides and Proteins, Academic Press, San Diego, CA, 1996, pp. 227-253.
F.W. Studier, A.H. Rosenberg, J.J. Dunn, J.W. Dubendorff, Use of T7 RNA polymerase to direct expression of cloned genes,Meth Enzymol185 (1990) 60-89.
Subramanian A., Immunoaffinity chromatography,Mol Biotechnol20 (2002) 41-47.
N.E. Thompson, D.B. Aronson, R.R. Burgess, Purification of eukaryotic RNA polymerase II by immunoaffinity chromatography. Elution of active enzyme with protein stabilizing agents from a polyol-responsive monoclonal antibody,J Biol Chem265 (1990) 7069-7077.
N.E. Thompson, D.A. Hager, R.R. Burgess, Isolation and characterization of a polyol-responsive monoclonal antibody useful for gentle purification of /Escherichia/ / coli/ RNA polymerase,Biochemistry31 (1992) 7003-7008.
N.E. Thompson, R.R. Burgess, Purification of recombinant human transcription factor IIB by immunoaffinity chromatography,Protein Exp Purif5 (1994) 468-475.
N.E. Thompson, L.A. Strasheim, K.M. Nolan, R.R. Burgess, Accessibility of epitopes on human transcription factor IIB in the native protein and in a complex with DNA,J Biol Chem270 (1995) 4735-4740.
N.E. Thompson, R.R. Burgess, Immunoaffinity purification of RNA polymerase II and transcription factors using polyol-responsive monoclonal antibodies,Meth Enzymol274 (1996) 513-26.
N.E. Thompson, R.R. Burgess, Identification of polyol-responsive monoclonal antibodies for use in immunoaffinity chromatography, in: F.M. Ausubel et al. (Eds) Current protocols in molecular biology, vol. 2, John Wiley & Sons Inc., New York, 2001, pp. 11-18.1.
N.E. Thompson, T.M. Arthur, R.R. Burgess, Development of an epitope tag for the gentle purification of proteins by immunoaffinity chromatography: application to epitope-tagged green fluorescent protein,Anal Biochem323 (2003) 171-179.
Thompson, N.E., Foley, K M, and Burgess, R.R. Antigen-binding properties of monoclonal antibodies reactive with human TATA-binding protein (TBP) and use in immunoaffinity chromatography.Prot. Express. Purific.In press, 2004.
R.Y. Tsien, The green fluorescent protein,Ann Rev Biochem67 (1998) 509-544.
Velander W.H., Orthner C.L., Thatakan J.P., Madurawe R.D., Ralston A.H., Strickland D.K., and drohan W.N., Process implications for metal-dependent immunoaffinity interactions.Biotechnol Prog, 5 (1989) 119-125.
Weller, M.G., Immunochromatographic techniques—a critical review,Fresenius J Anal Chem, 366 (2000) 635-645.
See website for Ne clone, Inc., Madison, Wisconsin: www.neoclone.com <http://www.neoclone.com/>.
Burgess Richard R.
Duellman Sarah J.
Thompson Nancy E.
DeWitt Ross & Stevens S.C.
Haq Shafiqul
Le Long V.
Leone, Esq. Joseph T.
Wisconsin Alumni Research Foundation
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