Immuno-PCR method for detecting nasopharyngeal carcinoma...

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving virus or bacteriophage

Reexamination Certificate

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Reexamination Certificate

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07807347

ABSTRACT:
The present invention is related to an immuno-PCR method for detecting nasopharyngeal carcinoma (NPC) and kit thereof, especially related to an immuno-PCR method for detecting markers of early stage NPC and kit thereof. The present invention includes providing a substrate whereon protein markers immobilized; applying a patient's specimen to the substrate; adding a solution which has biotinylated anti-human IgA secondary antibody and incubating the solution; adding a solution with a linker and biotinylated target DNA; proceeding a polymerase chain reaction; and finally, detecting the target DNA fragments via electrophoresis.

REFERENCES:
Piehler et al, Biosensors & Bioelectronics, 2000, vol. 15, pp. 473-481.
Tsang et al, Head & Neck, 2004, vol. 26(7), pp. 598-602.
Niemeyer et al,Trends in Biotechnology, 2005, vol. 23, No. 4, pp. 208-216.

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