Immuno-latex chromatographic procedure for detection of ciguatox

Chemistry: analytical and immunological testing – Involving an insoluble carrier for immobilizing immunochemicals – Carrier is particulate and the particles are of...

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436534, 436161, 436815, 435975, G01N 3312, G01N 33543, G01N 33546

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active

055255256

ABSTRACT:
An antibody test suitable for the rapid detection of ciguatoxin and other related low molecular weight polyether marine lipid toxins in fish tissue in the field or laboratory is disclosed. The test utilizes the reaction between antibody coated, mixed latex beads and any lipids in an extract of a sample of fish tissue eluted in a solvent. The presence of toxins is determined within about thirty minutes by ascending chromatography, which separates the mixed beads. The toxin concentration is determined by reference to standard data. A kit suitable for performing the testing procedure in the field is also disclosed. The kit includes a support having a nylon membrane covering, and antibody coated, mixed beads applied on the membrane, a testing container, a supply of solvent and a biopsy tool.

REFERENCES:
patent: D342031 (1993-12-01), Richardson
patent: 4816392 (1989-03-01), Hokama
patent: 5206141 (1993-04-01), Park
patent: 5238652 (1993-08-01), Sun et al.
patent: 5266497 (1993-11-01), Imai et al.
patent: 5286498 (1994-02-01), Park et al.
Bangs, L. B.: Latex agglutination tests. Amer. Clin. Lab News 7:(4A)20-25, 1988.
Bangs, L. B.: Latex immunoassays. J. Clin. Immunoassay 13:127-131, 1990.
Bangs, L. B.: New developments in particle-based tests and immunoassays. J. Int'l Fed. Clin. Chem. 2:(4), 188-192, 1990.
Hokama, Yoshitsugi: Simplified solid-phase immunobead assay for detection of ciguatoxin and related polyethers, J. Clin. Lab. Analy. 4:213-217, 1990.
Hokama, Y., Banner, A. H., Boyland, D. A.: A radioimmunoassay for detection of ciguatoxin. Toxicon 15:317-325, 1977.
Hokoma, Y., A rapid simplified enzyme immunoassay stick test for the detection of ciguatoxin and related polyethers from fish tissues. Toxicon 23:939-946, 1985.
Hokama, Y., Abad, M. A., Kimura, L. H.: A rapid enzyme immunoassay (EIA) for the detection of ciguatoxin in contaminated fish tissues. Toxin 21:817-824, 1983.
Hokama, Y., Honda S. A. A., Kobayashi, M. N., Nakagawa, L. K., Shirai, L. K., and Miyahara, J. T.: Monoclonal antibodies (MonoAbs) to ciguatoxin and related polyethers. In Lecture Notes on Coastal and Estuarine Studies vol. 25, Yentsch, C. M., Mague, F. C. and Horan, P. K. (eds.), Springer-Verlag, New York, pp. 155-166, 1988.
Hokama, Yoshitsugi: Recent methods for detection of seafood toxins: recent immunological methods for ciguatoxin and related polyethers, Food Additives and Contaminants, 10: (1) 71-82, 1993.
McHugh, T. M., Wang, Y. J., Chong, H.O., Blackwood, L. L., and Stites, D. P.: Development of a microsphere-based fluorescent immunoassay and its comparision to an enzyme immunoassay for the detection of antibodies to three antigen preparations from Candida albicans, J. Immunol. Meth., 116:213-219, 1989.

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