Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1997-07-10
1999-02-09
Housel, James C.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 6, 4241361, 530317, G01N 3353, G01N 33537, G01N 33543
Patent
active
058692746
ABSTRACT:
The embodiments of the invention are multiple antibody immunoassay systems, and preferably immunohistochemical staining systems. In these systems the detection antibody system that detects the association of a targeting antibody (that binds to the target moiety to be detected) may also cross-react with endogenous immunoglobulins in the specimen in which the target moiety is to be detected, yielding an unreliable assay with high background levels. This problem has been overcome by a process that includes cross-linking a monovalent antibody to the endogenous antigenic determinants prior to exposure to the targeting antibody and detecting antibody system. The embodiments of the invention also include kits for use in these multiple antibody immunoassay and histochemical staining systems.
REFERENCES:
patent: 4780406 (1988-10-01), Dolbeare et al.
patent: 4885237 (1989-12-01), Evans et al.
patent: 5084378 (1992-01-01), Crissman et al.
patent: 5219837 (1993-06-01), Cohen et al.
patent: 5334708 (1994-08-01), Chang et al.
patent: 5496549 (1996-03-01), Yamazaki et al.
patent: 5541287 (1996-07-01), Yau et al.
patent: 5571675 (1996-11-01), Baker et al.
patent: 5601819 (1997-02-01), Wong et al.
patent: 5643727 (1997-07-01), Reed et al.
Nielsen et al., "A method for the blocking of endogenous immunoglobulin on frozen tissue sections in the screening of human hybridoma antibody in culture supernatants" Hybridoma (1987) 6:103-109.
Foulds et al., "The use of fab fragments in a screening method for the detection of human anti-tumor monoclonal antibodies" Hybridoma (1988) 9:91-96.
Brandon, "Improved immunocytochemica staining through theuse of fab fragments of primary antibody, fab-specific second antibody, and fab-specific second antibody, and fab-horseradish peroxidsae" J of Histochemistry and Cytochemistry (1985) 33(7):715-719.
Burgess, et al. "Two-colour immunoenzymatic technique using sequential staining by APAAP to evaluate two cell antigens" J Clin Path (1992) 45:206-209.
Carayon, et al. "Identification of DNA-replicating lymphocyte subsets using a new method to label the bromo-deoxyuridine incorporated into the DNA" J of Immunological Methods (1992) 147:225-230.
Ffrench, et al. "Choice of fixation and denaturation for the triple labelling of intra-cytoplasmic antigen, bromodeoxyuridine and DNA" Histochemistry (1994) 101:385-390.
Foulds et al., "The use of fab fragments in a screening method for the detection of human anti-tumor monoclonal antibodies" Hybridoma (1988) 9:91-96.
Gratzner, et al. "The use of antibody specific for bromodeoxyuridine for the immunofluorescent determination of DNA replication in single cells and chromosomes" Experimental Cell Research (1975) 95:88-94.
Montuenga, et al. "Simultaneous immunostaining method for localization of bromodeoxyuridine and calcitonin gene-related peptide" J of Histochemistry and Cytochemistry (1992) 40(8):1121-1128.
Muir, et al. "An enzyme linked immunosorbent assay for bromodeoxyuridine incorporation using fixed microcultures" Analytical Biochemistry (1990) 185:377-382.
Negoescu, et al. "F(ab) secondary antibodies: A general method for double immunolabeling with primary antisera from the same species. Efficiency control by chemiluminescence" J of Histochemistry and Cytochemistry (1994) 42(3):433-437.
Nielsen et al., "A method for the blocking of endogenous immunoglobulin on frozen tissue sections in the screening of human hybridoma antibody in culture supernatants" Hybridoma (1987) 6: 103-109.
Soriano, et al. "Simultaneous immunocytochemical visualization of bromodeoxyuridine and neural tissue antigens" J of Histochemistry and Cytochemistry (1991) 39(3):255-263.
Toba et al., "Improved staining method for the simultaneous flow cytoflurometric analysis of DNA content, s-phase fraction, and surface phenotye using single laser instrumentation" Cytometry (1992) 13:60-67.
Giordano, M et al, 1991, Leukemia Res., vol. 15(11), pp. 965-974.
Carayon, P et al, J. of Immunol. Methods, vol. 147, 1992, pp. 225-230.
Soriano et al, J Histochem Cytochem, vol. 39, #3, pp. 255-263, 1991.
Montuenga et al, J Histochem Cytochem, vol. 40, #8, pp. 1121-1128, 1992.
Brandon, C, J Histochem Cytochem, vol. 33, #7, pp. 715-719, 1985.
Ffrench, et al, Histochemistry, vol. 101, pp. 385-390, 1994.
Toba et al, Cytometry, vol. 13, pp. 60-67. 1992.
Negoescu et al, J Histochem Cytochem, vol. 42, #3, pp. 433-437, 1994.
Muir et al, Analytical Biochem. vol. 185, pp. 377-382, 1990.
Carayon et al, J Immunolog Meth., vol. 147, pp. 225-230, 1992.
Burgess et al, J Clin. Path., vol. 45, pp. 206-209, 1992.
Gratzner et al, Exp. Cell Research, vol. 95, pp. 88-94, 1975.
Luu Peter
Shi Zuo-Rong
Tsao Dean
Housel James C.
Portner Ginny Allen
Zymed Laboratories, Inc.
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