Immuno gold lateral flow assay

Details Immuno gold lateral flow assay Immuno gold lateral flow assay

2011-03-22

2011-03-22

Nguyen, Bao-Thuy L (Department: 1641)

Chemistry: analytical and immunological testing

Involving diffusion or migration of antigen or antibody

C435S287700, C435S805000, C435S970000, C435S967000, C435S975000, C436S518000, C436S501000, C436S512000, C436S523000, C436S536000, C436S807000, C422S051000, C422S051000, C422S051000, C422S051000, C422S067000

Reexamination Certificate

active

07910381

ABSTRACT:
A chromatographic specific binding assay strip device, comprising: a non-permeable platform strip; a permeable membrane testing strip positioned on top of said non-permeable platform strip, with the testing strip comprising at least one capture reagent site containing a capture reagent for at least one specific analyte, a sample receiving pad positioned on top of and at a proximal end of the non-permeable platform strip, with the sample receiving pad having contact with a proximal end of said permeable membrane testing strip, a reservoir pad positioned on top of and at a distal end of said non-permeable membrane testing strip, with the reservoir pad having contact with a proximal end of said permeable membrane test strip; a supporting strip attached to and extending from the proximal end of said non-permeable platform strip; and a conjugate pad positioned on said supporting strip, said conjugate pad comprising a semi-permeable membrane containing a colorant conjugate. The semi-permeable membrane acts as a barrier between the conjugate pad and the sample receiving pad, regulating the flow through the semi-permeable membrane and overall flow of the assay by dipping the conjugate pad into a sample solution, there will be increased binding between the analyte in the sample and the conjugate (preferably colloidal gold), thereby giving improved results on the lateral flow assay.

REFERENCES:
patent: 5565366 (1996-10-01), Akers, Jr.
patent: 5602040 (1997-02-01), May et al.
patent: 6534324 (2003-03-01), Zin

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