Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1989-09-29
1992-03-03
Kepplinger, Esther L.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 38, 435975, 436548, 436808, 436815, G01N 33569, G01N 33577
Patent
active
050932357
ABSTRACT:
A novel immuno-dye reagent capable of detecting the presence of endotoxin in samples has been developed. The immuno-dye reagent comprises a solution of new methylene blue and an anti-endotoxin monoclonal antibody specific to a selected endotoxin. The immuno-dye reagent can be used in an assay to detect endotoxin by reacting the immuno-dye reagent with an endotoxin suspect pH adjusted sample, under hydrophobic conditions. The immuno-dye reagent can also be used in any application where binding of endotoxin is crucial, such as purifying endotoxin-contaminated solutions.
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Johnson et al., "JLN Endotoxin Reagent Assay for Endotoxemia", FASEB Conference, No. 3965, p. A977, Apr. 1988.
Lee et al., "Immunological Approaches in Septic Shock Research", Molecular and Cellular Mechanisms of Septic Shock, pp. 167-173, 1989.
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Kaplan et al., "Rapid Identification of the Invading Microorganism", Pediatric Clinics of North America, 27:783-803, 1980.
Johnson Akindele O.
Lee Che-Hung
Williams Taffy J.
Garvert William C.
Kepplinger Esther L.
Spevack A. David
The United States of America as represented by the Secretary of
Wolski Susan C.
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