Chemistry: analytical and immunological testing – Involving an insoluble carrier for immobilizing immunochemicals – Carrier is organic
Patent
1983-09-30
1986-04-15
Nucker, Christine M.
Chemistry: analytical and immunological testing
Involving an insoluble carrier for immobilizing immunochemicals
Carrier is organic
436531, 436532, 436533, 436534, 436548, 436823, 435181, G01N 33544, G01N 33545, G01N 33549, G01N 3353
Patent
active
045828105
ABSTRACT:
A suspension of diagnostic particles comprising antibody molecules attached to a carboxylate derivatized polymer core is provided for agglutination tests. The antibody is linked to the core through an avidin-biotin bridge. Avidin is joined by an amide bond to carboxyl groups on the core, and biotin is linked by an amide bond to amino groups on the antibody molecule. The core-bound antibody is exposed to a mixture of free biotin and biotinylated antibody to attach a controlled amount of antibody that is consistent with suspension stability prior to its use in a test and rapid cross-linking of suspended particles in the presence of antigen.
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Rappuoli et al., "Competitive Enzyme Immunoassay for Human Chorionic Somatomammotropin Using the Avidin-Biotin System", Analytical Biochemistry, 118, 168-172, (1981).
Guesdon et al., "The Use of Avidin-Biotin Interaction in Immunoenzymatic Techniques", The Journal of Histochemistry and Cytochemistry, vol. 27, No. 8, pp. 1131-1139, 1979.
Berman et al., Amplification of the Biotin-Avidin Immunofluorescence Technique", Journal of Immunological Methods, 36 (1980), pp. 335-338.
Becton Dickinson and Company
Nucker Christine M.
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