Immune complex transfer with lypophilic bridge

Details Immune complex transfer with lypophilic bridge Immune complex transfer with lypophilic bridge

1992-05-27

1994-05-17

Kepplinger, Esther L.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435961, 435968, 435971, 436528, 436540, 436 71, 436829, G01N 3392, G01N 33541, G01N 33544

Patent

active

053127307

ABSTRACT:
This invention relates to methods that have been found useful in reducing non-specific binding in immunochemical assays, via methods that can be implemented much faster than those used by Ishikawa. The techniques include the use of a lipophilic bridge, such as a liposome, or the elution of the antigen-antibody complex from the solid phase by the use of an anti-idiotypic antibody or an antibody, or the use of a heterologous reversible bridge.

REFERENCES:
patent: 4434236 (1984-02-01), Freytag
patent: 4828981 (1989-05-01), Maggio
patent: 4971904 (1990-11-01), Luddy
patent: 5009998 (1991-04-01), Chow
E. Ishikawa et al, Development and applications of ultrasensitive enzyme immunoassays for antigens and antibodies, 185 Clinica Chimica Acta (1989) 223.
T. Kohno et al, Measurement of Anti-Thyroglobulin IgG in Serum by Novel and Sensitive Immune Complex Transfer Enzyme Immunoassay, 22 Clin Biochem (1989) 277.

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