Immune complex isolation

Details Immune complex isolation Immune complex isolation

1988-03-21

1992-05-26

Nucker, Christine

Chemistry: analytical and immunological testing

For preexisting immune complex or auto-immune disease

436507, 436509, 436542, 530413, 5303871, 5303893, 424 88, G01N 33531, G01N 33564

Patent

active

051167662

ABSTRACT:
To diagnose diseases in patients, a protein complex, RhC, is prepared from horse serum by precipitating a white powder from the serum at a pH of 5.5 and processing to remove lipids at a pH of 8.2 using Tris-HCl as the buffer. It includes two components associated together to provide a molecular weight of 280,000 and having characteristics of a rheumatoid factor and a Clq-like subcomponent of the complement. The protein complex is incubated with human serum or plasma and then precipitated by dialysis against a high pH buffer (0.05M Tris-HCl pH 8.2). When precipitated, it co-precipitates the immune complexes from the human blood serum without substantial monomeric immunoglobulin to quantitatively isolate immune complexes from serum. Immunological assays then determine how much immune complex and what kind were in the serum.

REFERENCES:
patent: 4279995 (1981-07-01), Ricci

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