Immune-chemical measurement process for haptens and proteins

Details Immune-chemical measurement process for haptens and proteins Immune-chemical measurement process for haptens and proteins

1985-09-13

1987-06-02

Nucker, Christine M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435 28, 435810, 436518, 436536, 436538, 436539, 436540, 436824, 436808, 422 61, G01N 3353, G01N 33543, G01N 33537, G01N 33541

Patent

active

046703837

ABSTRACT:
This invention describes a process for immuno-chemical quantitative determination of immunologically active substances. The method involves a first incubation of a sample containing the active substance with a labelled binder, which contains an antibody or antibody fragment. After complexing has taken place, solid phase bound active substance identical to the substance being quantitatively determined is added. The solid phase bound substance binds with free binder, and the solid and liquid phases are separated. A second antibody which is specific either to antibody or the solid phase antibody-substance complex is then added to the liquid phase. This second antibody is non cross-reactive with individual complex components. The amount of labelled first antibody bound to second antibody is then determined.

REFERENCES:
patent: 3720760 (1973-03-01), Bennich et al.
patent: 4048298 (1977-09-01), Niswender

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