Immumoassays and kit for detection of proteoglycans

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

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435 6, 435 792, 435810, 436501, 5303871, 5303875, 5303882, G01N 3353, G01N 33543, C07K 1700

Patent

active

051852454

ABSTRACT:
The invention discloses methods of detecting proteoglycans in synovial fluid and methods of monitoring treatment of diseases characterized by breakdown of proteoglycans into synovial fluid. A test sample of synovial fluid is contacted with antibodies specifically bindable with proteoglycan, the antibodies being bound to a solid support. Bound proteoglycan is then contacted with detectably labeled antibody specifically bindable with said proteoglycan and the detectable label is then detected.

REFERENCES:
patent: 4391904 (1983-07-01), Litman et al.
patent: 4704356 (1987-11-01), Thonar
patent: 4778768 (1988-10-01), Heinegard et al.
Witter et al., "The Immunologic Detection and Characterization of Cartilage Proteoglycan Degradation Products In Synovial Fluids of Patients With Arthritis", Arthritis and Rheumatism, 30: 519-529 (1987).
Saxne, et al., Annals of the Rheumatic Diseases, 45:491-497 (1986).
Sampson, et al., Analytical Biochemistry, 151:304-308 (1985).
Heimer, et al., Analytical Biochemistry 165:448-455 (1987).

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