Multicellular living organisms and unmodified parts thereof and – Nonhuman animal
Patent
1995-02-28
1997-10-07
Chambers, Jasemine C.
Multicellular living organisms and unmodified parts thereof and
Nonhuman animal
4351723, 43524026, 43524027, 935 70, 935 89, 935 93, 935106, C12N 1500, C12N 512, C12N 1506, C12N 524
Patent
active
056750633
ABSTRACT:
Novel methods are provided for producing rabbit fusion partners for the production of specific binding proteins, particularly immunoglobulins. Nuclei of rabbit fertilized ova are microinjected with an oncogene to express specifically in lymphoid cells (i.e., lymphoid specific) and the embryos are then implanted in appropriate hosts. The newborns are maintained to sexual maturity and are either mated with rabbit hosts that developed from embryos that had been microinjected with a different lymphoid-specific oncogene or their fertilized eggs are microinjected with another oncogene specific for lymphoid cells. The immortalized lymphoid cells that develop in these animals may be genetically altered and used as fusion partners to produce rabbit hybridomas for the production of monoclonal antibodies.
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Chambers Jasemine C.
Hauda Karen M.
Loyola University of Chicago
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