Chemistry: molecular biology and microbiology – Animal cell – per se ; composition thereof; process of... – Primate cell – per se
Patent
1995-07-20
1998-02-10
Ketter, James
Chemistry: molecular biology and microbiology
Animal cell, per se ; composition thereof; process of...
Primate cell, per se
4353201, C12N 510, C12N 1585, C12N 1586
Patent
active
057168458
ABSTRACT:
This invention is directed to construction of recombinant plasmids that contain the Epstein-Barr virus ("EBV") genetic information involved in the immortalization of human lymphocytes, but that lack one or more genes implicated in EBV lytic replication. The recombinant plasmids have the advantage of being able to immortalize human lymphocytes, while being incapable of generating infectious EBV particles. The invention also provides human lymphocytes that have been successfully infected and immortalized by the recombinant plasmids. These human lymphocyte clones can be used as cellular factories to produce desired proteins and as delivery vehicles for gene therapy. Additionally, the invention establishes a method by which desired proteins can be produced in immortalized cellular factories, without generating infectious EBV particles. These proteins can be the normal cellular products of immortalized human lymphocytes, or they can be proteins encoded by foreign genes, which have been cloned into the lymphocytes.
REFERENCES:
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Hammerschmidt, Wolfgang et al., Genetic analysis of immortalizing functions of Eptstein-Barr virus in human B lymphocytes, Reprinted from Nature, vol. 340, No. 6232, Aug. 1989, pp. 393-397.
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Kempkes, Bettina et al., Immortalization of Human B Lymphocytes by a Plasmid Containing 71 Kilobase Pairs of Epstein-Bar Virus DNA, Journal of Virology, vol. 69, No. 1, Jan. 1995, pp. 231-238.
Kieff, Elliott et al., Epstein-Barr Virus and Its Replication, Virology, Second Edition, edited by B. N. Fields, D. M. Skipe et al., Raven Press, Ltd., New York .COPYRGT.1990, pp. 1889-1920.
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Hammerschmidt Wolfgang Friedrich
Sugden William Maxwell
Ketter James
Wisconsin Alumni Research Foundation
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