Immortalized human skin cell lines and novel serum-free...

Chemistry: molecular biology and microbiology – Animal cell – per se ; composition thereof; process of... – Primate cell – per se

Reexamination Certificate

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C435S325000, C435S366000, C435S467000

Reexamination Certificate

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06949381

ABSTRACT:
The present invention relates to improved continuous (immortalized) cell lines, in particular keratinocytes and melanocytes derived from normal human skin tissue. The present invention also relates to novel serum-free media for isolating, producing and maintaining said improved continuous keratinocyte and melanocyte cell lines. The present invention also relates to methods for producing primary melanocytes and keratinocytes under serum-free conditions without any feeder cells.

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patent: 6423540 (2002-07-01), Baur et al.
patent: 196 17 261 (1996-11-01), None
patent: WO 95/27510 (1995-10-01), None
P. Boukamp et al., “Normal Keratinization in a Spontaneously Immortalized Aneuploid Human Keratinocyte Cell Line”,The Journal of Cell Biology, 106:761-771 (1988).
A. Pfeifer et al., “Highly efficient establishment of immortalized cells from adult human liver”,Methods on Cell Science, 17:83-89 (1995).
M. Pittelkow et al., “New Techniques for the In Vitro Culture of Human Skin Keratinocytes and Perspectives on Their Use for Grafting of Patients With Extensive Burns”,Mayo Clin Proc, 61:771-777 (1986).
H. Navsaria et al., “Culturing skin in vitro for wound therapy”,TIBTECH13:91-100 (1995).
E. Sbano et al., “Pemphigus Antibodies Fixation and Keratinocyte Differentiation in Organ Cultures”,Arch Dermatol Res, 273:9-14 (1982).
V. Steinkraus et al., “High density of beta2-adrenoceptors in a human keratinocyte cell line with complete epidermal differentiation capacity (HaCaT)”,Arch Dermatol Res, 283:328-332 (1991).
Derwent WPI Acc No: 97-022262/199703, English language abstract of Germany 196 17 261.

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