Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1998-07-13
1999-11-23
Wortman, Donna C.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435347, 435371, G01N 33567, C12N 508
Patent
active
059898372
ABSTRACT:
A spontaneously immortalized human keratinocyte cell line is disclosed. In a preferred embodiment, this cell line is ATCC 12191. In another embodiment of the invention, a method of assaying the effect of a test tumor cell modulation agent is disclosed. The method comprises the steps of obtaining a human stratified squamous epithelial cell culture, wherein the culture comprises human malignant squamous epithelial cells and spontaneously immortalized human keratinocytes, wherein the culture forms a reconstituted epidermis. One then treats the epidermis with a test tumor cell modulation agent and evaluates the growth of the malignant cells within the epidermis.
REFERENCES:
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J.A, Garlick, "Skin Substitutes--Tissue Models for Cancer Biology," Bioengineering of Skin Substitutes, Sep. 18-19, Boston, MA, 1997.
R.H. Rice, et al., "Elevation of Cell Cycle Control Proteins During Spontaneous Immortalization of Human Keratinocytes," Mol. Biol. Cell 4:185-194, 1993.
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Allen-Hoffmann et al., Polycyclic hydrocarbon mutagenesis of human epidermal keratinocytes in culture, Proc. Natl. Acad. Sci. USA 81:7802-7806, 1984.
Allen-Hoffmann B. Lynn
Pickart Michael A.
Schlosser Sandra J.
Wisconsin Alumni Research Foundation
Wortman Donna C.
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