Chemistry: molecular biology and microbiology – Spore forming or isolating process
Patent
1993-05-19
1996-04-09
Fleisher, Mindy
Chemistry: molecular biology and microbiology
Spore forming or isolating process
435 6, C12N 510
Patent
active
055061311
ABSTRACT:
Non-tumorigenic, stable, human bronchial and liver epithelial cell lines are provided wherein the cell lines are capable of expressing human cytochrome P450 genes which have been inserted into the cell lines. Also provided are methods and kits for identifying potential mutagens, cytotoxins, carcinogens, chemotherapeutic and chemo-preventive agents utilizing these cell lines.
REFERENCES:
patent: 4675002 (1987-06-01), Viles et al.
patent: 4816395 (1989-03-01), Hancock
patent: 4885238 (1989-12-01), Reddel
patent: 5164313 (1992-11-01), Gelboin
patent: 5356806 (1994-10-01), Harris et al.
Nebert et al., DNA and Cell Biol., vol. 10, 1991, pp. 1-14.
Bayad et al., Biochem. Pharmacol., vol. 42, 1991, pp. 1345-1351.
Harris et al., Cancer Research, vol. 47, 1987, pp. 1-10.
U.S. Ser. No. 07/377,967, filed Dec. 19, 1989 by Cole et al.
Lechner et al., "Non-tumorigenic human liver epithelial cell cultures for chemical and biological carcinogenesis investigations," J. Cell. Biochemistry Supp. 15D, p. 140 (1991).
Crespi et al., "Stable expression of human cytochrome P450IA2 cDNA in a human lymphoblastoid cell line: role of the enzyme in the metabolic activation of aflatoxin B.sub.1," Molecular Carcinogenesis, 3(1):5-8 (1990).
Crespi et al., "A metabolically competent human cell line expressing five cDNAs encoding procarcinogen-activating enzymes: application to mutagenicity testing," Chem. Res. Toxicol., 4(5):566-572 (Oct. 1991).
Mace et al., "Increased activation of promutagens in a human bronchial epithelial cell line stably expressing the human cytochrome P450 IA2," Proceedings of the 83rd Annual Meeting of the American Association for Cancer Research, Abstract No. 945; vol. 33, Mar. 1992, May 20-23, 1992, San Diego, CA.
Arthur I. Grayzel, et al., "The Effects of Inhibitors Of Protein Synthesis On The Synthesis of Heme In Rabbit Reticulocytes," Biochemical and Biophysical Research Communications, 28(5) (1967).
R. C. Garner and A. E. M. McLean, "Separation of HAEM Incorporation From Protein Synthesis In Liver Microsomes," Biochemical and Biophysical Research Communications, 37(6):883-887 (1969).
K. W. Bock and P. Siekevitz, "Turnover Of HEME And Protein Moieties Of Rat Liver Microsomal Cytochrome b.sub.5," Biochemical and Biophysical Research Communications, 41(2):374-380 (1970).
Manford K. Patterson, Jr., "Measurement of Growth and Viability of Cells in Culture," Methods in Enzymology, 58:141-152 (1979).
Harry V. Gelboin, "Benzo[.alpha.]pyrene Metabolism, Activation, and Carcinogenesis: Role and Regulation of Mixed-Function Oxidases and Related Enzymes," Physiological Reviews, 60(4):1107-1166 (1980).
Gwen S. Duthu, et al., "Characterization of NADPH-cytochrome P-450 reductase in a mouse hepatoma cell line," Biol. Abstr., 76(10):#73560 (1983).
John F. Lechner, et al., "Differential Control by Platelet Factors of Squamous Differentiation in Normal and Malignant Human Bronchial Epithelial Cells," Cancer Research, 43:5915-5921 (1983).
Shioko Kimura, et al., "The Murine Ah Locus," The Journal of Biological Chemistry, 259(17):10705-10713 (1984).
Sekhar Chakrabarti, et al., "Vaccinia Virus Expression Vector: Coexpression of .beta.-Galactosidase Provides Visual Screening of Recombinant Virus Plaques," Molecular and Cellular Biology, 5(12):3403-3409 (1985).
Pietro Ghezzi, et al., "Role of reactive oxygen intermediates in the interferon-mediated depression of hepatic drug metabolism and protective effect of N-acetylcysteine in mice," Biological Abstracts, 80(12):AB-961:#91194 (1985).
John F. Lechner and Moira A. LaVeck, "A Serum-Free Method For Culturing Normal Human Bronchial Epithelial Cells At Clonal Density," Journal of Tissue Culture Methods, 9(2):43-48 (1985).
Kenji Oeda, et al., "Expression of Rat Liver Cytochrome P-450MC cDNA in Saccharomyces cerevisiae," DNA, 4(3):203-210 (1985).
Hiroyuki Sadano and Tsuneo Omura, "Incorporation of Heme to Microsomal Cytochrome P-450 in the Absence of Protein Biosynthesis," J. Biochem., 98(5):1321-1331 (1985).
William T. Wickner and Harvey F. Lodish, "Multiple Mechanisms of Protein Insertion Into and Across Membranes," Science, 230:400-406 (1985).
George H. Yoakum, et al., "Transformation of Human Bronchial Epithelial Cells Transfected by Harvey ras Oncogene," Science, 227:1174-1178 (1985).
Philippe H. Beaune, et al., "Isolation and sequence determination of a cDNA clone related to human cytochrome P-450 nifedipine oxidase," Proc. Natl. Acad. Sci. USA, 83:8064-8068 (1986).
Anil K. Jaiswal, et al., "Human P.sub.3 450: cDNA and complete amino acid sequence," Nucleic Acids Research, 14(16):6773-6774 (1986).
Y. Ke, et al., "Human Bronchial Epithelial Cells Transformed by SV40 Gene(s) Retain the Ability to Undergo Terminal Squamous Differentiation," J. Cell Biol., 103(2): 27a:#95 (1986).
M. Mackett and G. L. Smith, "Vaccinia Virus Expression Vectors," J. gen. Virol., 67:2067-2082 (1986).
Tohru Masui, et al., "Growth and Differentiation of Normal and Transformed Human Bronchial Epithelial Cells," Journal of Cellular Physiology Supplement, 4:73-81 (1986).
Toshitaro Nakagawa, et al., "Sodium N-Butyrate Modulates The Transcription And Posttranscriptional Processing of the Calcitonin Gene in Human Medullary Thyroid Carcinoma," Federation Proceedings, 46:718:#2352 (1987).
R. R. Reddel, et al., "Human Mesothelial Cells Are Transformed By Transfection With SV40 Large T Antigen Gene," J. Cell Biol., 103(2): 27a:#94 (1986).
Byung-Joon Song, et al., "Complementary DNA and Protein Sequences of Ethanol-inducible Rat and Human Cytochrome P-450s," The Journal of Biological Chemistry, 261(35):16689-16697 (1986).
Edward B. Stephens, et al., "Surface expression of viral glycoproteins in polarized in epithelial cells infected with recombinant vaccinia viral vectors," Biol. Abstr., 81(12):AB-560:#112680 (1986).
Mauricio X. Zuber, et al., "Expression of Bovine 17.alpha.-Hydroxylase Cytochrome P-450 cDNA in Nonsteroidogenic (COS 1) Cells," Science, 234:1259-1261 (1986).
Narayana Battula, et al., "Expression of P.sub.1 -450 and P.sub.3 -450 DNA coding sequences as enzymatically active cytochromes P-450 in mammalian cells," Proc. Natl. Acad. Sci. USA, 84:4073-4077 (1987).
Douglas E. Brash et al., "Strontium Phosphate Transfection of Human Cells in Primary Culture: Stable Expression of the Simian Virus 40 Large-T-Antigen Gene in Primary Human Bronchial Epithelial Cells," Molecular and Cellular Biology, 7(5):2031-2034 (1987).
B. I. Gerwin, et al., "Growth Factor Production By Normal Human Mesothelial Cells and Mesothelioma Cell Lines," European Association for Cancer, p. 1752 (1987).
Brenda Gerwin, et al., "Response To Growth Factors By Normal Human Mesothelial Cells And Their Autocrine Production By Human Mesothelioma Cell Lines," J. Cell. Biochem., Supplement 11, Abstract A217, p. 58 (1987).
Shioko Kimura, et al., "cDNA and amino acid sequences of two members of the human P450IIC gene subfamily," Nucleic Acids Research, 15(23):10053-10054 (1987).
J. F. Lechner, et al., "Normal Human Mesothelial Cells Are Responsive To Unusual Combinations Of Growth Factors," Proceedings of AACR, 28:60:#240 (1987).
R. Reddel, et al., "Development of Non-Tumorigenic Human Mesothelial Cell Lines With Transfected SV40 Large T Antigen Gene," European Association for Cancer, p. 1794 (1987).
R. R. Reddel, et al., "Establishment of Non-Tumorigenic Human Bronchial Epithelial Cell Lines," Proc. Amer. Assoc. Cancer Res., 28:118:#469 (1987).
J. S. Rhim, et al., "An In Vitro Multi-Step Model for Human Epithelial Cell Carcinogenesis," Proc. Amer. Assoc. Cancer Res., 28:120:#475 (1987).
Sasajima, et al., "Effects of tumor promoters and cocarcinogens on growth and differentiation of cultured human esophageal epithelial cells," J. National Cancer Institute, 78(3):419-423 (1987).
Diane R. Umbenhauer, et al., "Cloning and Sequence Determination of a Complementary DNA Related to Human Liver Microsomal Cytochrome P-450 S-Mephenytoin 4-Hydroxylase," Biochemistry, 26:1094-1099 (1987).
Paul Amstad, et al,. "Neoplastic Transformation of a Human Bronchial Epithelial Cell Line by a Recombinant Retrovirus Encoding Viral Harvey ras,
Gelboin Harry V.
Gonzalez Frank J.
Harris Curtis C.
Mace Katharine C.
Pfeifer Andrea M. A.
Fleisher Mindy
Ketter James
The United States of America as represented by the Department of
LandOfFree
Immortalized human cell lines containing exogenous cytochrome P4 does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Immortalized human cell lines containing exogenous cytochrome P4, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Immortalized human cell lines containing exogenous cytochrome P4 will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-138238