Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving fixed or stabilized – nonliving microorganism,...
Patent
1998-11-25
2000-07-25
Park, Hankyel
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving fixed or stabilized, nonliving microorganism,...
536 2372, 435 6, 435 691, 435325, C12Q 108, C12Q 168, C07H 2104, C12P 2106, C12N 500
Patent
active
060935537
ABSTRACT:
A brain microvessel endothelial cell having a Middle-T antigen gene from papovirus is disclosed. The brain microvessel endothelial cell exhibits normal cell phenotype, maintains the phenotype in culture and forms monolayers substantially impermeable to low molecular weight molecules. The cells are useful for in vitro studies involving the blood brain barrier. Also disclosed, are methods of using and processes of making these cells.
REFERENCES:
patent: 5674722 (1997-10-01), Mulligan et al.
patent: 5733761 (1998-03-01), Treco et al.
Durieu-Trautmann, O., Immortalization of Brain Capillary Endothelial Cells with Maintenance of Structural Characteristics of the Blood-Brain Barrier Endothelium, In Vitro Cell Dev. Biol., Oct. 1991 pp. 771-777.
DeBault, Lawrence E., Cerebral Microvessels and Derived Cells in Tissue Culture: Establishment, Identification and Preliminary Characterization of an Endothelial Cell Line, In Vitro vol.k 17, No. 6, Jun. 1981, pp. 480-494.
Vicart, Patrick, Immortalization of Multiple Cell Types from Transgenic Mice Using a Transgene Containing the Vimentin Promoter and a Conditional Oncogene, Experimental Cell Research 214, pp. 35-45.
Roux, F. Regulation of Gamma-Glutamyl Transpeptidase and Alkaline Phosphatase Activities in Immortalized Rat Brain Microvessel Endothelial Cells, Journal of Cellular Physiology 159:101-113 (1994).
Aguzzi, Adriano, Cell type-specific tumor induction in neural transplants by retrovirus-mediated oncogene transfer, Oncogene (1991),6, 113-118.
Treisman, R., Transformation of rat cells by an altered polyoma virus genome expressing only the middle-T protein, Nature vol. 292, Aug. 13, 1981, pp. 595-600.
Soeda, Eiichi; Coding potential and regulatory signals of the polyoma virus genome, Nature vol. 283, Jan. 31, 1980, 445--453.
Juillerat-Jeanneret, L.; Dexamethasone Selectively Regulates the Activity of Enzymatic Markers of Cerebral Endothelial Cell Lines; In Vitro Cel Dev. Bio.--Jul.-Aug. 1992, pp. 537-543.
Bormann Barbara J.
Yazdanian Mehran
Boehringer Ingelheim Pharmaceuticals Inc.
Bottino Anthony P.
Park Hankyel
Raymond Robert P.
Stempel Alan R.
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