Immortalized and malignant human prostatic cell lines

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

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435 721, 435371, 435975, 435366, 436 63, 436 64, G01N 33574, G01N 33567, G01N 3353, C12N 500

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058244883

ABSTRACT:
Immortalized malignant human prostatic epithelial and fibroblast cell lines are described containing DNA of a human papillomavirus (HPV) and similar cell lines containing DNA of a human papillomavirus and an activated viral ras oncogene, such as v-Ki-ras. The cell lines are useful for research on drugs for treatment of prostatic cancer and other diseases. The cell lines are useful for research on causes, treatment and prevention of prostate cancer, benign prostatic hyperplasia, male infertility, birth defects, aging and assessment of environmental toxic agents.

REFERENCES:
Rhim et al: Neoplastic transformation of human epidermal keratinocytes by AD12-SX40 and Kirsten Saroma Virus, Science: 227, 1250-1252, 1985.
Samid et al: Selective growth arrest and phenotypic reversion of prostate cancer cells in vitro by nontoxic pharmacological concentrations of phenylacetate. The J. of Clin. Investigation, 91:2288-2295, 1993.
Rhim et al: Human Popillomavirus type 18 DNA immortalizes normal adult human prostate cells, Proceeding of the American Association for Cancer Research, vol. 34, p. 117 1993.
Cussenot, O.: Immortalization of human adult normal prostatic epithelial cells by liposomes containing large T-SV40 gene. J. of Urol., vol. 143 pp. 881-886, 1991.
Kaur et al: Characterization of primary human keratinocytes transformed by human papillomavirus type 18. J. of Virol. of Virol. vol. 62, pp. 1917-1924, 1988.

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