Chemistry: molecular biology and microbiology – Process of mutation – cell fusion – or genetic modification – Fusion of cells
Reexamination Certificate
2007-05-29
2007-05-29
Schnizer, Richard (Department: 1635)
Chemistry: molecular biology and microbiology
Process of mutation, cell fusion, or genetic modification
Fusion of cells
C435S346000, C435S357000, C435S363000, C435S377000, C435S325000, C435S320100, C435S455000
Reexamination Certificate
active
09604876
ABSTRACT:
The present invention provides an immortalized human cardiomyocyte cell line. The present invention further provides a method for preparing a human immortalized cell line derived from a post-mitotic primary cell culture.
REFERENCES:
Watanabe et.al.; Cardiomyocyte Transplantation in a Porcine Myocardial Infarction Model, 1998, Cell Transplantation, vol. 7, No. 239-246.
Koh et.al.; Strategies for Myocardial Repair, 1995, Journal of Interventional Cardiology, vol. 8, No. 4: 387-393.
Wang et al (In Vitro Cellular and Developmental Biology 27(1): 63-74, Jan. 1991). See abstract.
Makino et al (J. Clin. Invest 103(5): 697-705(1999).
Leiden (J. Clin. Invest 103(5): 591-592(1999)).
Connan et al (Nature (1985) 314(6008): 277-279).
Claycomb, W., Lanson, N., Stallworth, B., Egeland, D.B., Delcaprio J.B., Bahinski, A. and Izzo, N. (1998). HL-1 cells: a cardiac muscle cell line that contracts and retains phenotypic characteristics of adult cardiomyocyte. PNAS. 95:2979-2984.
King, M., Koga, Y., Davidson, M. and Schon E. (1992). Defects in mitochondrial protein synthesis and respiratory chain activity segregate with the tRNALeu(UUR) mutation associated with mitochondrial myopathy, encephalopathy, lactic acidosis and stroke like episodes. Mol. Cell. Biol. 12:480-490.
King, M. and Attardi, G. (1989). Human cells lacking mtDNA: repopulation with exogenous mitochondria by complementation. Science 246:500-503.
Singer, K., Scearce, R.M., Tuck, D.T. Whichard, L.P., Denning S.M. and Haynes B.F. (1989). Removal of fibroblasts from human epithelial cell cultures with use of a complement fixing monoclonal antibody reactive with human fibroblasts and monocytes/macrophages. J.Invest.Dermatol.92:166-170.
Bader, D., et al., “Immunochemical analysis of myosin heavy chain during avian myogenesisin vivoandin vitro.” J. Cell. Biol., 95:763-770 (1982) (Exhibit 1).
Bloch, K.D., et al., “Neonatal atria and ventricles secrete atrial natriuretic factors via tissue-specific secretory pathways.”Cell, 47:695-702 (1986) (Exhibit 2).
Brunskill, E.W., et al., “Novel cell lines promote the discovery of genes involved in early heart development.”Dev. Biol., 235:507-520 (2001) (Exhibit 3).
Campion, D.R., “The muscle satellite cell: a review.”Int. Rev. Cytol., 87:225-51 (1984) (Exhibit 4).
Cantin, M., et al., “The heart as an endocrine gland.”J. Hypertens., 2 (Suppl. 3):329-331 (1984) (Exhibit 5).
Chiu, R.C., et al., “Cellular cardiomyoplasty: myocardial regeneration with satellite cell implantation.”Ann. Thorac. Surg., 60:12-18 (1995) (Exhibit 6).
Claycomb, W.C., “Atrial-natriuretic-factor mRNA is developmentally regulated in heart ventricles and actively expressed in cultured ventricular cardiac muscle cells of rat and human.”Biochem. J., 255:617-620 (1988) (Exhibit 7).
Claycomb, W.C., et al., “Culture of the terminally differentiated adult cardiac muscle cell: a light and scanning electron microscope study.”Dev. Biol., 80:466-482 (1980) (Exhibit 8).
Constantin, B., et al., “Involvement of gap junctional communication in myogenesis.”Int. Rev. Cytol., 196:1-65 (2000) (Exhibit 9).
de Bold, A.J., “Atrial natriuretic factor: a hormone produced by the heart.”Science, 230:767-770 (1985) (Exhibit 10).
Delorme, B., et al., “Expression pattern of connexin gene products at the early developmental stages of the mouse cardiovascular system.”Circ. Res., 81:423-437 (1997) (Exhibit 11).
Doevendans, P.A., et al., “Differentiation of cardiomyocytes in floating embryoid bodies is comparable to fetal cardiomyocytes.”J. Mol. Cell. Cardiol., 32:839-851 (2000) (Exhibit 12).
Eppenberger-Eberhardt, M., et al., “New occurrence of atrial natriuretic factor and storage in secretorially active granules in adult rat ventricular cardiomyocytes in long-term culture.”J. Mol. Cell. Cardiol., 25:753-757 (1993) (Exhibit 13).
Eppenberger-Eberhardt, M., et al., “Reexpression of α-smooth muscle actin isoform in cultured adult rat cardiomyocytes.”Dev. Biol., 139:269-278 (1990) (Exhibit 14).
Fabrizi, G.M., et al., “Differential expression of genes specifying two isoforms of subunits Vla of human cytochrome c oxidase.”Gene, 119:307-312 (1992) (Exhibit 15).
Franke, W.W., et al., “Specific immunohistochemical detection of cardiace/fetal α-actin in human cardiomyocytes and regenerating skeletal muscle cells.”Differentiation, 60:245-250 (1996) (Exhibit 16).
Fürst, D.O., et al., “Myogenesis in the mouse embryo: differential onset of expression of myogenic proteins and the involvement of titin in myofibril assembly.”J. Cell. Biol., 109:517-527 (1989) (Exhibit 17).
Goldman, B.I., et al., “Human fetal cardiocytes in enriched culture.”In Vitro Cell. Dev. Biol. Anim., 31:731-734 (1995) (Exhibit 18).
Graef, I.A., et al., “NFAT signaling in vertebrate development.”Curr. Opin. Genet. Dev., 11:505-512 (2001) (Exhibit 19).
Hescheler, J., et al., “Establishment of ionic channels and signaling cascades in the embryonic stem cell-derived primitive endoderm and cardiovascular system.”Cells Tissues Organs, 165:153-164 (1999) (Exhibit 20).
Horsley, V., et al., “NFAT: ubiquitous regulator of cell differentiation and adaptation.”J. Cell. Biol., 156(5):771-4 (2002) (Exhibit 21).
Jaenicke, T., et al., “The complete sequence of the human β-myosin heavy chain gene and a comparative analysis of its product.”Genomics, 8:194-206 (1990) (Exhibit 22).
Jaffredo, T., et al., “MC29-immortalized clonal avian heart cell lines can partial differentiatein vitro.” Exp. Cell Res., 192:481-491 (1991) (Exhibit 23).
Janssen, P.M., et al., “Preservation of contractile characteristics of human myocardium in multi-day cell culture.”J. Mol. Cell. Cardiol., 31:1419-1427 (1999) (Exhibit 24).
Katz, E.B., et al., “Cardiomyocyte proliferation in mice expressing α-cardiac myosin heavy chain-SV40 T-antigen transgenes.”Am. J. Physiol., 262:H1867-H1876 (1992) (Exhibit 25).
Koga, Y., et al., “Sequence of a cDNA specifying subunit VIIc of human cytochrome c oxidase.”Nucleic Acids Res., 18(3):684 (1990) (Exhibit 26).
Li, R., et al., “Human pediatric and adult ventricular cardiomyocytes in culture: assessment of phenotypic changes with passaging.”Cardiovasc. Res., 32:362-373 (1996) (Exhibit 27).
Litzkas, P., et al., “Efficient transfer of cloned DNA into human diploid cells: protoplast fusion in suspension.”Mol. Cell. Biol., 4(11):2549-2552 (1984) (Exhibit 28).
Lloyd, T.R., et al., “Sympathetic innervation improves the contractile performance of neonatal cardiac ventricular myocytes in culture.”J. Mol. Cell. Cardiol., 22:333-342 (1990) (Exhibit 29).
Lyons, G.E., et al., “Developmental regulation of myosin gene expression in mouse cardiac muscle.”J. Cell. Biol., 111:2427-2436 (1990) (Exhibit 30).
Lyons, G.E., et al., “The expression of myosin genes in developing skeletal muscle in the mouse embryo.”J. Cell. Biol., 111:1465-1476 (1990) (Exhibit 31).
Marvin, W.J., Jr., et al., “Correlation of function and morphology of neonatal rat and embryonic chick cultured cardiac and vascular muscle cells.”Circ. Res., 45:528-540 (1979) (Exhibit 32).
Molkentin, J.D., “The zinc finger-containing transcription factors GATA-4, -5, and -6. Ubiquitously expressed regulators of tissue-specific gene expression.”J. Biol. Chem., 275:38949-52 (2000) (Exhibit 33).
Murry, C.E., et al., “Skeletal myoblast transplantation for repair of myocardial necrosis.”J. Clin. Invest., 98(11):2512-2523 (1996) (Exhibit 34).
Negishi, Y., et al., “Multipotency of a bone marrow stromal cell line, TBR31-2, established from ts-SV40 T antigen gene transgenic mice.”Biochem. Biophys. Res. Commun., 268:450-455 (2000) (Exhibit 35).
Polinger, I.S., “Separation
Cooper & Dunham LLP
Schnizer Richard
The Trustees of Columbia University in the City of New York
White, Esq. John P.
LandOfFree
Immortalization of human post-mitotic cells does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Immortalization of human post-mitotic cells, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Immortalization of human post-mitotic cells will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3797232