Chemistry: molecular biology and microbiology – Spore forming or isolating process
Patent
1995-06-07
1997-09-30
Leguyader, John L.
Chemistry: molecular biology and microbiology
Spore forming or isolating process
435 691, 4351723, 4353201, C12Q 102, C12N 1585
Patent
active
056724993
ABSTRACT:
The invention includes mammalian multipotent neural stem cells and their progeny and methods for the isolation and clonal propagation of such cells. At the clonal level the stem cells are capable of self regeneration and asymmetrical division. Lineage restriction is demonstrated within developing clones which are sensitive to the local environment. The invention also includes such cells which are transfected with foreign nucleic acid, e.g., to produce an immortalized neural stem cell. The invention further includes transplantation assays which allow for the identification of mammalian multipotent neural stem cells from various tissues and methods for transplanting mammalian neural stem cells and/or neural or glial progenitors into mammals. A novel method for detecting antibodies to neural cell surface markers is disclosed as well as a monoclonal antibody to mouse LNGFR.
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Reynolds, B.A., et al., "EG
Anderson David J.
Stemple Derek L.
California Institute of Technology
LeGuyader John L.
Silva Robin M.
Trecartin Richard F.
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