Immobolized light emitting systems

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving luciferase

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23230B, 435175, 435176, 435177, 435178, 435181, C12Q 166, C12N 1118, C12N 1114, C12N 1110

Patent

active

042346819

ABSTRACT:
Light emitting systems are immobilized on a support to provide a localized, concentrated release of light that increases the sensitivity of detection systems when assaying bio-materials. A preferred immobilized light emitting system contains an oxidoreductase and luciferase bound to glass beads that are attached to an elongated glass rod.

REFERENCES:
patent: 3817837 (1974-06-01), Rubenstein et al.
patent: 3940250 (1976-02-01), Plakas et al.
Erlanger, et al., Insoluble Bacterial Luciferases: A New Approach to Some Problems in Bioluminescence, Biochemical and Biophysical Research Comm. vol. 40, No. 1 1970 (pp. 70-76).
Picciolo, et al., Applications of Luminescent Systems to _Intectidus Diseased Methodology, Goddard Space Flight Center, Greenbelt, Maryland, 1976 (pp. 1-4).
Waters, et al., Flavin binding by Bacterial Luciferase: Affinity Chromatography of Bacterial Luciferase, Biochem. Biophys. Res. Comm., vol. 57, No. 4, 1974 (pp. 1152-1158).
Hommerstedt, R. H. An Automated Method for ATP Analysis Utilizing the Luciferi-Luciferase Reaction Analytical Biochemistry, vol. 52, 1973 (pp. 449-455).
Brolin, et al., Photokinetic Micro Assay Based on Dehydrogenase Reactions and Bacterial Luciferase, Analytical Biochemistry. vol. 42, 1971 (pp. 124-135).
Stanley P. E., Determination of Subpicomole Levels of NADH and FMN Using Luciferase and the Liquid Scintillation Spectrometer, Analytical Biochemistry, vol. 39, 1971 (pp. 441-453).
Weetall, H. H., Immobilized Enzymes, Antigens, Antibodies and Peptides, Marcel Dekker, Inc. N.Y., 1975 (pp. 17, 18 & 74-80).
Weetall H., Applications of Immobilized Enzymes, Analytical Chemistry, vol. 48 No. 7, 6, 1976 (pp. 544A-548A, 550A, 554A, 556A, 557A & 558A).

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