Chemistry: molecular biology and microbiology – Carrier-bound or immobilized enzyme or microbial cell;... – Enzyme or microbial cell is immobilized on or in an organic...
Patent
1988-11-04
1991-06-04
Naff, David M.
Chemistry: molecular biology and microbiology
Carrier-bound or immobilized enzyme or microbial cell;...
Enzyme or microbial cell is immobilized on or in an organic...
435135, 435182, 435198, 435280, C12N 1108, C12N 920, C12P 762, C07C 6700
Patent
active
050213458
ABSTRACT:
Lipase is immobilized by mixing a substrate, a crude porcine pancreatic lipase, an aqueous buffer solution at pH 5-9 and one or more water-soluble polyhydric aliphatic alcohols having from 2 to 6 carbon atoms and from 2 to 6 hydroxyl groups at a temperature of from 0.degree. to 40.degree. C. to form a mixture, allowing the mixture to stand for from 1 minute to 3 days, filtering the mixture to produce a filter cake and washing the cake to obtain the immobilized lipase. The substrate is a pulverulent, insoluble, only slightly swellable copolymer of one or more N-vinyllactams of 4 to 6 carbon atoms with a cyclic amide which contains two or more ethylenically unsaturated, copolymerizable groups, one or more of which are bonded directly to amide nitrogen. The polyhydric aliphatic alcohol can be combined with one or more water-soluble monohydric alcohols having from 1 to 4 carbon atoms. The filter cake can be treated with a crosslinking agent such as a water-soluble glycidyl ether which is prepared by reacting an epoxide with a polyfunctional alcohol to form an adduct and reacting the adduct with epichlorohydrin followed by cyclization to give an epoxide. The immobilized lipase is used for resolving a racemate of an ester of a racemic alcohol such as a racemate of glycidyl butyrate.
REFERENCES:
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patent: 4732853 (1988-03-01), Whitesides et al.
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patent: 4798793 (1989-01-01), Eigtued
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Applied Biochemistry & Microbiology, vol. 14, Nr. 5, Sep./Oct. 1978, "Immobilization . . . Epoxy Groups".
Journal of American Chemical Society 1984, 106, pp. 7250-7251 Lipase-Catalyzed Hydrolysis as a Route to Esters of chiral Epoxy Alcohols, Wolfgang Ladner, et al.
Ladner Wolfgang
Paul Axel
Urban Dieter
BASF - Aktiengesellschaft
Naff David M.
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