Immobilizing microbial cells with polyfunctional aziridines

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound having a 1-thia-4-aza-bicyclo

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435 52, 435 94, 435108, 435109, 435116, 435180, 435182, C12P 3700, C12P 3300, C12N 1108, C12N 1104

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active

046507558

ABSTRACT:
Microbial cells are immobilized with a curable polyaziridine or polyfunctional aziridine prepolymer to obtain an insoluble, crosslinked polymer containing the cells. The microbial cells immobilized may be cells having L-aspartase or L-phenylalanine transaminase activity for the production of L-aspartic acid or L-phenylalanine. The polymer containing the cells may be formed as a coating on a solid inert carrier.

REFERENCES:
Porath et al., Methods in Enzymology, vol. XLIV, 1976, pp. 19-45.
Chibata, I., Immobilized Enzymes, John Wiley & Sons, N.Y., 1978, pp. 14-27, 50-55 & 72-81.

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