Immobilizing and processing specimens on matrix materials for th

Details Immobilizing and processing specimens on matrix materials for th Immobilizing and processing specimens on matrix materials for th

1998-04-14

2000-08-15

Stucker, Jeffrey

Chemical apparatus and process disinfecting, deodorizing, preser

Analyzer, structured indicator, or manipulative laboratory...

435 6, 435 71, 436518, 436810, G01N 2100

Patent

active

061031925

ABSTRACT:
The invention is a method and device for collecting and processing a biological specimen for the analyses of nucleic acids. A device comprises a matrix to which cells and viruses adhere and a handle to manipulate the matrix. The devices are used to collect, dry, transport, store and process small amounts of blood or other tissue. The matrix of the device is transferred to a reaction tube and amplifying reagents added to it. Nucleic acid sequences and relative quantities are detected and analyzed from the same specimen. The relative amounts of amplified nucleic acid from one or more particular RNA sequences are compared to one another and to the amount of amplified nucleic acid from DNA sequences serving as an internal control for the number of biological units per specimen. The relative amounts of amplified viral sequences from suspected viruses in the biological specimen and from recombinant viral particles serving as a viral quantitation standard enable estimation of viral burden in a given quantity of specimen.

REFERENCES:
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Nucleic Acids Research 23:3788-9, 1995, Makowski et al., "Enhanced Direct Amplification of Guthrie Card DNA Following Selective Elution of PCR Inhibitors".
J. Clinical Microbiology 31: 1364-7, 1993, Yourno, J., Direct Polymerase Chain Reaction for Detection of Human Immunodeficiency Virus.
ISO Code.TM. PCR DNA Sample Isolation Device, 1995 and ISO Code.TM. Stix PCR Template Preparation Dipsticks, 1996 Schleicher & Schuell, Keene, NH, USA & Dassel, Germany.

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