Immobilized restriction endonucleases

Details Immobilized restriction endonucleases Immobilized restriction endonucleases

1978-04-17

1982-08-03

Naff, David M.

Chemistry: molecular biology and microbiology

Carrier-bound or immobilized enzyme or microbial cell;...

Enzyme or microbial cell is immobilized on or in an organic...

435 91, 435172, 435174, 435176, 435179, 435180, 435199, C12N 1110, C12N 922, C12P 1934

Patent

active

043428339

ABSTRACT:
Restriction endonucleases are immobilized by chemically bonding the restriction endonucleases to water-insoluble matrices. The immobilized restriction endonucleases have enhanced stability and can be used to obtain deoxyribonucleic acid fragments in relatively low salt media, free of protein and ready for further use without tedious and time-consuming manipulations.

REFERENCES:
patent: 3909358 (1975-09-01), Stanley et al.
patent: 4013514 (1977-03-01), Wildi et al.
patent: 4039382 (1977-08-01), Thang et al.
Roberts, R. J., Restriction Endonucleases, Critical Reviews in Biochemistry, Nov. 1976 (pp. 123, 151, 152, 153 & 163).
Zaborsky, O., Immobilized Enzymes, CRC Press, Cleveland, Ohio, 1973 (pp. 2, 30-32, 128, 129 &134-138).

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