Immobilized microbial nitrilase for production of glycolic acid

Details Immobilized microbial nitrilase for production of glycolic acid Immobilized microbial nitrilase for production of glycolic acid

2007-10-31

2010-06-22

Fronda, Christian L (Department: 1652)

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Stablizing an enzyme by forming a mixture, an adduct or a...

C435S069100, C435S183000, C435S227000, C536S023200

Reexamination Certificate

active

07741088

ABSTRACT:
The present invention is directed to a process for improving the specific activity of a dehydrated enzyme catalyst having nitrilase activity for hydrolysis of glycolonitrile to glycolic acid upon rehydration. In particular, a process is provided comprising pretreating an enzyme catalyst having nitrilase activity with glutaraldehyde, immobilizing the gutaraldehyde-preteated enzyme catalyst and chemically cross-linking the enzyme catalyst prior to dehydration. Upon rehydration, the enzyme catalyst exhibits improved specific nitrilase activity as compared to enzyme catalysts having nitrilase activity that are dehydrated and rehydrated without the processing described herein.

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