Immobilized microbial cell composition for making L-aspartic aci

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing alpha or beta amino acid or substituted amino acid...

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435176, 435179, 435180, 435182, C12P 1320, C12N 1112, C12N 1108, C12N 1104

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active

044368135

ABSTRACT:
Microbial cells having L-aspartase activity are immobilized for producing L-aspartic acid. The cells are immobilized by mixing the cells with a curable prepolymer material and curing the prepolymer material to form a crosslinked polymer. Suitable prepolymer materials are polyazetidine prepolymers, carboxymethyl cellulose which can be crosslinked with polyvalent ions, polyurethane hydrogel prepolymers and polymethylene isocyanates. A preferred prepolymer material is polyazetidine prepolymer. The polymer may be formed as a coating on a solid inert carrier.

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patent: 4237229 (1980-12-01), Hartdegen et al.
Fusee et al., Immobilization of Escherichia coli, Cells Containing Aspartase Activity with Polyurethane and Its Application for L-Aspartic Acid Production, Applied and Environmental Microbiology, vol. 42, No. 4, 1981, (pp. 672-676).
Hustad et al., Immobilization of Beta-Galactosidase on an Insoluble Carrier with a Polyisocyanate Polymer.I Preparation and Properties, J. Da. Sci., vol. 56, No. 9, 1973, (pp. 1111-1117).

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