Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing alpha or beta amino acid or substituted amino acid...
Patent
1982-03-16
1984-03-13
Naff, David M.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing alpha or beta amino acid or substituted amino acid...
435176, 435179, 435180, 435182, C12P 1320, C12N 1112, C12N 1108, C12N 1104
Patent
active
044368135
ABSTRACT:
Microbial cells having L-aspartase activity are immobilized for producing L-aspartic acid. The cells are immobilized by mixing the cells with a curable prepolymer material and curing the prepolymer material to form a crosslinked polymer. Suitable prepolymer materials are polyazetidine prepolymers, carboxymethyl cellulose which can be crosslinked with polyvalent ions, polyurethane hydrogel prepolymers and polymethylene isocyanates. A preferred prepolymer material is polyazetidine prepolymer. The polymer may be formed as a coating on a solid inert carrier.
REFERENCES:
patent: 3791926 (1974-02-01), Chibata et al.
patent: 3860490 (1975-01-01), Guttag
patent: 4138292 (1979-02-01), Chibata et al.
patent: 4237229 (1980-12-01), Hartdegen et al.
Fusee et al., Immobilization of Escherichia coli, Cells Containing Aspartase Activity with Polyurethane and Its Application for L-Aspartic Acid Production, Applied and Environmental Microbiology, vol. 42, No. 4, 1981, (pp. 672-676).
Hustad et al., Immobilization of Beta-Galactosidase on an Insoluble Carrier with a Polyisocyanate Polymer.I Preparation and Properties, J. Da. Sci., vol. 56, No. 9, 1973, (pp. 1111-1117).
Calton Gary J.
Wood Louis L.
Naff David M.
Purification Engineering, Inc.
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