Immobilized metal aqueous two-phase extraction and precipitation

Details Immobilized metal aqueous two-phase extraction and precipitation Immobilized metal aqueous two-phase extraction and precipitation

1992-08-05

1994-02-01

Killos, Paul J.

Organic compounds -- part of the class 532-570 series

Organic compounds

Heterocyclic carbon compounds containing a hetero ring...

548108, 548403, 556114, 556134, 556148, 562448, 562557, 562564, 562568, 562571, 562575, 564505, C07F 108, C07F 300, C07F 1500

Patent

active

052833399

ABSTRACT:
Metallated compounds can be used to extract or precipitate proteins from solution. The compounds can be polymers, such as polyalkylene glycols, which can be mono- or bimetallated or smaller bis-metal chelates, such as ethylenebis(oxyethylenenitrilo)tetraacetic acid. The monometallated polymers are preferred in the extraction process whereas the bimetallated compounds are preferred in the precipitation process. In addition, new PEG compounds which are capable of chelating a variety of metals are described. Both mono- and bi-metallated forms are set forth. The new metallated compounds are very effective in extracting or precipitating proteins from solution.

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Dust, Julian M. et al. "Proton NMR Characterization of Poly(ethylene glycols) and Derivatives", Macromolecules, vol. 23, pp. 3742-3746 (1990).
Van Dam, Mariana E. et al. "Metal Affinity Precipitation of Proteins", Biotechnology and Applied Biochemistry, vol. 11, pp. 492-502 (1989).
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