Immobilized metal, affinity chromatography

Details Immobilized metal, affinity chromatography Immobilized metal, affinity chromatography

1998-01-12

1999-08-03

Therkorn, Ernest G.

Liquid purification or separation

Processes

Liquid/liquid solvent or colloidal extraction or diffusing...

210656, 530413, 530417, B01D 1508

Patent

active

059321024

ABSTRACT:
A method for purifying protein containing histidine residues using immobilized metal, affinity chromatography. The hydrophilic index of the histidine residues is determined (HI). If the HI is at least 2 the pH of the solution containing the protein is adjusted to about 6.75 to 7.2 and applied to the IMAC column such that the protein binds to the column.

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Porath et al., 1983, Affintiy Chromatography and Biological Recognition, 173-89 (Academic Press, San Diego).

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