Immobilized luciferase for the quantitative determination of ATP

Details Immobilized luciferase for the quantitative determination of ATP Immobilized luciferase for the quantitative determination of ATP

1988-04-01

1989-05-23

Naff, David M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving luciferase

435174, 435177, 530354, C12Q 166, C12N 1100, C12N 1102

Patent

active

048330752

ABSTRACT:
Luciferase is immobilized on a membrane prepared from an absorbent albuminoid such as procine skin gelatin. The membrane may be formed by spreading a solution of porcine skin gelatin on a support and after incubation and drying removing the resultant membrane from the support. Preferably, the membrane is crosslinked with a solution of glutaraldehyde, and luciferase is immobilized by saturating the membrane with luciferase in the presence of dithiothreitol. The immobilized luciferase is particularly suitable for the quantitative determination of adenosine triphosphoric acid (ATP).

REFERENCES:
patent: 4411999 (1983-10-01), Shigesada et al.
patent: 4601981 (1986-07-01), Vieth et al.
Romette, et al., Cinica Chimica Acta., vol. 95, 1979, pp. 249-253.
Kricka, et al., Trac, Trends in Analytical Chemistry, vol. 2, No. 11, 1983, pp. 244-247.
Ugarora, et al., Chemical Abstracts, 97:87825x, 1982, p. 366.
The Condensed Chemical Dictionary, 8th ed., VHR, N.Y., 1971, pp. 412-413.

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